Verification of the Interaction between SET and eEF1A1 in Human Liver Cells by Co-immunoprecipitation  

利用免疫共沉淀技术验证SET与eEF1A1在人肝细胞内的相互作用(摘要)(英文)

作　　者：杨亮[1,2] 杨细飞[2] 张毅[1] 刘建军[2] 李杰[2] 

机构地区：[1]华南理工大学生物科学与工程学院,广东广州510006 [2]深圳市疾病预防控制中心毒理研究室,广东深圳518020

出　　处：《Agricultural Science & Technology》2010年第8期87-90,共4页农业科学与技术（英文版）

基　　金：Supported by National Natural Science Foundation (30972454);Shenzhen Science and Technology Plan Major Project (200801010)~~

摘　　要：[Objective] The aim was to investigate the possible interaction between SET and eEF1A1 in human liver cells. [Method] Firstly the total proteins of human L-02 liver cells were extracted under non-denaturing conditions; then,mouse anti-human SET and rabbit anti-human eEF1A1 antibodies were used to perform the co-immunoprecipitation respectively; subsequently,the immunoprecipitations was correspondingly detected with rabbit anti-human eEF1A1 and mouse anti-human SET antibodies by Western Blot. [Result] EEF1A1 was detected in protein complex from the immunoprecipitations by using anti-SET antibody,and SET also was detected in immunoprecipitations by using anti-eEF1A1 antibody. [Conclusion] The interaction between SET and eEF1A1 in human liver cells was confirmed.[目的]探讨SET与eEF1A1在人肝细胞内是否存在相互作用。[方法]以人L-02肝细胞为材料,在非变性条件下提取总蛋白,将鼠抗人SET、兔抗人eEF1A1抗体分别加到总蛋白提取物中,用偶联了琼脂糖珠的ProteinA/G分离抗原抗体复合物,最后分别用兔抗人eEF1A1、鼠抗人SET抗体进行Western blotting检测。[结果]在用SET抗体免疫共沉淀下来的蛋白复合物中检测到eEF1A1,同时在用eEF1A1抗体免疫共沉淀下来的蛋白复合物中也检测到SET。[结论]SET与eEF1A1在人肝细胞内存在相互作用。

关 键 词：SET eEF1A1 IMMUNOPRECIPITATION INTERACTION 

分 类 号：R341[医药卫生—基础医学]