Cloning and Expression Analysis of the High Mobility B Group Genes in Arabidopsis thaliana  

拟南芥高迁移率族蛋白B(HMGB)族基因的克隆及表达分析(摘要)(英文)

作　　者：冀芦沙[1] 王洪霞[1] 郭尚敬[1] 

机构地区：[1]聊城大学生命科学学院,山东聊城252059

出　　处：《Agricultural Science & Technology》2010年第8期160-163,172,共5页农业科学与技术（英文版）

摘　　要：[Objective] The aim was to better research the function and action mode of High Mobility Group B （HMGB） proteins in higher plants. [Method] At2G33450,At5G23405 and At5G23420 genes of HMGB protein family in Arabidopsis thaliana were cloned by the use of RT-PCR method,and the expression of these three proteins in E.coli and Arabidopsis thaliana were detected by using SDS-PAGE,Northern blot and subcellular localization methods. [Result] The results showed that the molecular weights of the three proteins were 17.5,17.0 and 27.0 kD respectively,and the expression levels of the proteins in Arabidopsis thaliana were At5G23420At5G23405At2G33450. In addition,all the three proteins were located in nucleus. [Conclusion] The study will provide a basis for the further research on the biological function of HMGB proteins in higher plants.[目的]为研究高等植物高迁移率族蛋白B家族的功能及作用模式。[方法]运用RT-PCR方法克隆出拟南芥HMGB蛋白家族中的3个基因:At2G34450、At5G23405、At5G23420,并运用SDS-PAGE、Northern检测及亚细胞定位等手段检测这3种蛋白在大肠杆菌及拟南芥中的表达。[结果]经鉴定,以上3种蛋白的分子量分别为17.5、17.0和27.5KD,在拟南芥中表达量At5G23420>At5G23405>At2G34450,且这3种蛋白均定位于细胞核内。[结论]该研究结果为深入研究高等植物中HMGB家族蛋白的生物学功能奠定了基础。

关 键 词：Arabidopsis thaliana High Mobility Group B （AtHMGB） Prokaryotic expression Subcellular localization Northern blot 

分 类 号：Q943.2[生物学—植物学]