高危型HPV病毒含量与宫颈癌前病变程度的关系研究  

Analysis of the relationship between high risk HPV virus and the severity of cervical precancerous lesions

作　　者：张劲丰[1] 安宏亮[1] 

机构地区：[1]广东省佛山市中医院检验科,广东佛山528000

出　　处：《实验与检验医学》2010年第6期535-536,542,共3页Experimental and Laboratory Medicine

基　　金：佛山市卫生局科研课题;课题号:2004416

摘　　要：目的分析高危型人乳头瘤病毒与子宫颈癌前病变的关系。方法选择来我院宫颈病-宫颈癌筛查防治中心就诊的892例病例,运用第二代基因杂交捕获信号放大技术(Hybrid CaptureⅡ,HC-Ⅱ)检测高危型HPV DNA含量,且同时行阴道镜病理活检。按病理结果将病例分为正常组、CIN-Ⅰ组、CIN-Ⅱ～Ⅲ组。以对数Log10转换原始的RLU/CO结果作为HR-HPV DNA的定量结果。统计各病例组阳性结果的病毒含量分布,验证数据的频数分布规律。应用SPSS 13.0软件一元线性回归分析HR-HPVDNA病毒含量与CIN病变程度的关系。结果 HR-HPV DNA阳性率分别是:正常组32.3%(223/693),CIN-Ⅰ53.7%(66/123)、CIN-Ⅱ为78.7%(37/47)、CIN-Ⅲ为93.1%(27/29)。各组阳性病例HPV DNA定量的均值和95%置信区间分别是:正常组1.56、1.42～1.70;CIN-Ⅰ组1.67、1.43～1.91;CIN-Ⅱ～CIN-Ⅲ组1.76、1.55～1.97,子宫颈癌前病变程度与HPV病毒DNA含量不呈线性关系(P>0.05)。结论高危型HPV感染与宫颈癌前病变的发生相关,并随病变程度的加重,感染率增高;HPV病毒含量的高低,不代表CIN病变的严重程度,然而,病毒含量越高,患高度病变的可能性越大。Objective To analyze the relationship between viral load of high risk type human papillomavirus（HR-HPV） and the severity of cervical precancerous lesions.Methods High risk HPV DNA content from 892 cases were detected by Hybrid Capture II（HC-Ⅱ）,and their pathological results were divided into the groups of normal,CIN-Ⅰ and CIN-Ⅱ～Ⅲ.By performing a logarithmic transformation,the original RLU/CO results were modified as viral load（lg RLU/CO）.The positive results of all viral load were calculated,and the frequency distribution was verified.The linear regression was applied to analyze the relationship between HR-HPV DNA viral load and severity of CIN lesions.Results HR-HPV DNA positive rates were as follows： normal group 32.3%（223/693）,CIN-Ⅰ 53.7%（66/123）,CIN-Ⅱ 78.7%（37/47）,and CIN-Ⅲ 93.1 %（27/29） respectively.HR-HPV DNA contents of each group（mean and 95% confidence interval） were normal group 1.56,1.42～1.70;CIN-Ⅰ group 1.67,1.43～1.91;CIN-Ⅱ～Ⅲ Group 1.76,1.55～1.97,respectively.DNA load and cervical lesions were not linearly related（P0.05）.Conclusions High risk HPV infection is related to the occurrence of cervical precancerous lesions,and its infectious rate increased with the severity of cervical precancerous lesions.The high HR-HPV visal load does not mean the severity of CIN lesions.However the higher the virus content,the greater risk of severe lesions.

关 键 词：人乳头瘤病毒 子宫颈上皮内瘤样变 基因杂交信号放大技术 

分 类 号：R446.8[医药卫生—诊断学] R737.33[医药卫生—临床医学]