银杏过氧化物酶基因POD1的克隆及表达分析  

Molecular Cloning,Characterization and Expression of POD1 Gene from Ginkgo biloba L.

作　　者：程华[1,2] 李琳玲[1] 王燕[3] 程水源[1] 

机构地区：[1]黄冈师范学院生命科学与工程学院,湖北黄冈438000 [2]南京林业大学森林资源与环境学院,江苏南京210037 [3]长江大学园艺园林学院,湖北荆州434025

出　　处：《华北农学报》2010年第6期44-51,共8页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金项目(30971974);湖北省自然科学基金计划重点项目(2008CDA061)

摘　　要：利用RACE技术从银杏中克隆到过氧化物酶基因(GbPOD1,FJ599670)的cDNA全长。GbPOD1基因编码序列为1 092 bp,编码一条长为329Aa的成熟阴离子POD蛋白,预测成熟蛋白分子量为35.8 kDa,等电点为8.10。Southern杂交及进化树分析结果表明,GbPOD1和其他物种的POD源自于相同的祖先,属于植物类型III的POD同工酶编码基因,为多基因家族。RT-PCR分析表明,GbPOD1在银杏的根、茎、叶和果中都有表达,在茎中的相对表达量最高,其次为根和果,而叶部位表达水平最低。植物激素MeJA、金属离子Cu和Cd以及MV和WOU的处理能显著诱导GbPOD1基因的表达。研究结果显示,在银杏叶片中,GbPOD1属于多功能基因,具有潜在的参与重金属污染清除及伤害处理防御方面的功能。The full-length cDNA sequence of novel peroxidase1 gene（designed as GbPOD1,NCBI accession number：FJ599670） were isolated from Ginkgo biloba L.The coding region of the gene is 1 092 bp long,and its deduced mature protein consists of 329 amino acids with a predicted molecular mass of 35.8 kDa and a pI of 8.10.Phylogenetic tree analysis revealed that GbPOD1 shared the same ancestor with other PODs,could be classified as type III peroxidase.The result of southern analysis showed that GbPOD1 gene was encoded by a multi-gene family in Ginkgo biloba.The expression analysis by RT-PCR showed that GbPOD1 expressed in a tissue-specific manner in G.biloba.GbPOD1 was also found to be up-regulated by MeJA,CuSO4,CdCl2,MV,and mechanical wounding.These results indicate that the GbPOD1 has the potential to play a role in response to heavy metal and wounding against foreign attacks,was a multifunction gene.

关 键 词：银杏 POD1基因 表达分析 

分 类 号：Q78[生物学—分子生物学]