磷脂酶C特异性抑制剂U-73122对玉米大斑病菌分生孢子萌发及毒素活性的影响  

Effects of Phospholipase C Inhibitor U-73122 on Conidial Germination and HT-toxin Activity of Setosphaeria turcica

作　　者：郝志敏[1] 郝杰[1] 李志勇[2] 董金皋[1] 

机构地区：[1]河北农业大学分子植物病理学实验室,河北保定071001 [2]河北省农林科学院谷子研究所,河北石家庄054000

出　　处：《华北农学报》2010年第6期118-121,共4页Acta Agriculturae Boreali-Sinica

基　　金：河北省自然科学基金资助项目(C2008000335)

摘　　要：磷脂酶C(Phospholipase C,PLC)通过降解细胞膜上的磷脂分子产生IP3和DAG两个第二信使分子,分别激活下游的Ca2+途径和蛋白激酶C。研究表明,PLC与植物病原真菌的致病及发育过程有关。利用PLC特异性抑制剂U-73122初步研究了玉米大斑病菌PLC的功能。结果显示,U-73122对玉米大斑病菌菌丝生长及分生孢子的萌发均具有明显的抑制作用,且抑制率随抑制剂浓度升高而增强;并且可引起芽管的缩短和不规则膨大。此外,U-73122也可以导致病菌粗毒素的生物学活性明显降低。由此说明PLC可以通过影响菌丝及芽管的极性生长,而调控玉米大斑病菌的菌丝及分生孢子发育;同时参与病菌的毒素合成。Phospholipase C（PLC）could catalyze the hydrolysis of phospholipid molecules on cell membrane and generate two second messenger molecules IP3 and DAG,which activated Ca2＋ signal pathway and protein kinase C respectively.It had been proved that PLC modulated pathogenicity and development of plant pathogenic fungi.In this research,U-73122,the specific inhibitor of PLCs,was used to study functions of PLCs in Setosphaeria turcica preliminary.U-73122 showed significant inhibition effects on hyphal growth and conidial germination.The higher was the inhibitor concentration,the lower was the germination ratio of spores.U-73122 caused the germ tube shortening and cell swelling irregularly.In addition,U-73122 could also obviously decrease the biological activity of HT-toxin.It was indicated that PLC might regulate hyphal growth and conidial germination by controlling the cell polarity,and also involved in the biosynthesis of HT-toxin in Setosphaeria turcica.

关 键 词：玉米大斑病菌 磷脂酶C U-73122 分生孢子 毒素 

分 类 号：S432.44[农业科学—植物病理学]