振脾汤对慢性非特异性溃疡性结肠炎大鼠肠组织中Fas、FasL及Survivin表达的影响  

Influence of Zhenpi decoction on Fas、FasL and Survivin expression of colon tissue in chronic nonspecific ulcerative colitis

作　　者：孔磊[1] 杨晓峰[1] 万玉良 赵增仁[2] 张学明[2] 

机构地区：[1]中国石油天然气集团公司中心医院普外科,河北廊坊065000 [2]河北医科大学附属第一医院普外科,河北石家庄050031

出　　处：《河北中医》2010年第12期1866-1869,F0003,共5页Hebei Journal of Traditional Chinese Medicine

基　　金：2008年廊坊市科学技术研究与发展指导计划(第一批)项目(编号:2008056101)

摘　　要：目的观察振脾汤对慢性非特异性溃疡性结肠炎(CUC)大鼠肠组织中Fas、FasL及Survivin表达的影响。方法 Wistar大鼠48只随机分为4组,对照组、模型组、中药组、柳氮磺胺吡啶(SASP)组,各12只。对照组用0.9%氯化钠注射液灌肠1次,其余4组三硝基苯磺酸(TNBS)/乙醇法制作CUC动物模型。制模2周后开始,对照组和模型组继续饲养4周;中药组予振脾汤水煎液2 mL(含生药17 g)灌胃,SASP组给予柳氮磺胺吡啶2 mL(含药0.25 g)灌胃,均日1次,连续2周,给药后继续饲养2周。制模6周后处死各组大鼠取肛门至回盲部结肠标本,卵白素-生物素-酶复合物(ABC)免疫组织化学染色法观察各组结肠标本Fas、FasL及Survivin的表达情况。结果模型组、中药组及SASP组Fas蛋白表达阳性细胞率明显高于对照组(P<0.05);中药组与模型组比较Fas蛋白表达明显下调(P<0.05),SASP组与模型组比较则无明显变化(P>0.05)。模型组、中药组及SASP组FasL蛋白表达的阳性细胞率与对照组比较均无明显变化(P>0.05),中药组、SASP组与模型组比较均无明显变化(P>0.05)。模型组、SASP组Survivin蛋白表达阳性细胞率低于对照组(P<0.05)。中药组与对照组的Survivin蛋白表达的阳性细胞无明显变化(P>0.05),而SASP组与对照组比较则有较明显变化(P<0.05);中药组、SASP组与模型组比较均无明显变化(P>0.05)。结论振脾汤能够有效地下调CUC大鼠的结肠黏膜腺上皮细胞中的Fas蛋白表达水平,从而抑制CUC大鼠的结肠黏膜腺上皮细胞的过度凋亡。Objective To observe the influence of Zhenpi decoction on Fas、FasL and Survivin expression of intestine tissue in chronic nonspecific ulcerative colitis.Methods 48 Wistar rats were randomly divided into four groups,control group,model group,traditional Chinese medicine group,Sulfasalazine group.Chronic ulcerative colitis rats model were established by TNBS/ethanol method.Control group,model group,traditional Chinese medicine group,Sulfasalazine group received 0.9% NaCl solution,Zhenpi decoction（2 mL extracted from 17 g crude Chinese herbs,once a day） and Sulfasalazine（0.25 g/2 mL,once a day）,respectively.Expression of Fas、FasL and survivin in rats＇ colon tissue of each group were detected by immunohistochemistry technique（ABC） after six weeks of establishing model.Results The rate of positive cells which express Fas in model group,traditional Chinese medicinal group and Sulfasalazine group was significantly higher than that of control group（P0.05）.Comparing with model group,traditional Chinese medicine group could apparently decrease the expression of Fas（P0.05）.Comparing with model group,Sulfasalazine group has no significant change in positive cells which express Fas（P0.05）.Comparing with control group,the rate of positive cells which express FasL of model group,traditional Chinese medicinal group and Sulfasalazine group have no obvious change（P0.05）.Comparing with model group,the rate of positive cells which express FasL of traditional Chinese medicinal group and Sulfasalazine group have no obvious change（P0.05）.Positive cells which express survivin of model group and Sulfasalazine group is much less than that of control group（P0.05）.The positive cells which express survivin of traditional Chinese medicinal group has no obvious change comparing with control group（P0.05）.The positive cells which express survivin of Sulfasalazine group has obvious change comparing with control group（P0.05）.The positive cells which express survivin of traditional Chinese medicine

关 键 词：结肠炎 溃疡性 中药疗法 慢性病 汤剂 FAS FASL Survivin 

分 类 号：R289.5[医药卫生—方剂学] R574.620.531[医药卫生—中药学]