百合查尔酮合成酶(chs)基因的cDNA克隆与分析  

Cloning and Sequencing of Chalcone Synthase Gene cDNA inLilium

作　　者：杨丽[1] 刘雅莉[2] 王跃进[2] 

机构地区：[1]信阳农业高等专科学校,河南信阳464000 [2]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《河南农业科学》2011年第1期124-126,共3页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(30371013)

摘　　要：根据报道的查尔酮合成酶基因的保守序列,设计了特异简并引物,以东方百合Sorbonne盛开的花瓣总RNA为模板,逆转录合成cDNA第一链,用特异引物对此双链cDNA进行PCR扩增,将扩增后的基因片段回收克隆后,送至上海博亚生物公司测序,目的序列长873 bp,与已发表的百合中CHS基因的同源性达到91%,说明克隆的片段属于查尔酮合成酶基因,GenBank上登陆号为DQ471950。The specific primers synthesized according to conserved regions of reported CHS gene were used to acquire CHS cDNA by RT-PCR.A fragment of about 870bp was obtained and the sequencing results showed that： the fragment length was 873bp and its sequence was over 91% homology to other reported CHS.The Genbank accession number was DQ471950.

关 键 词：百合 查尔酮合成酶 CDNA 克隆 

分 类 号：S644.1[农业科学—蔬菜学]