HPLC法测定妇炎舒丸中芍药苷含量  

Determintion of paeoniflorin of Fuyanshu Wan by HPLC

作　　者：齐晓飞[1] 张伏[1] 张璐[1] 赵晓冬[1] 

机构地区：[1]辽宁省食品药品检验所,沈阳110023

出　　处：《实用药物与临床》2011年第1期43-44,共2页Practical Pharmacy and Clinical Remedies

摘　　要：目的建立高效液相色谱法,测定妇炎舒丸中芍药苷的含量。方法采用Hypersol C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-水(20:80),检测波长为230 nm,流速1.0 mL/min,柱温为30℃。结果芍药苷在0.395 2～4.742 4μg范围内线性关系良好,r=0.999 9,平均回收率为95.60%,RSD为0.14%(n=9)。结论本方法简便、准确、重现性好,适用于该药品成分的含量测定。Objective To establish an HPLC method for determining paeoniflorin of Fuyanshu Wan.MethodsThe HPLC system consisted of Hypersol C18 column,HCN-H2O（20：80） mixture as a mobile phase,detecting wave-length at 230 nm.The flow rate was 1.0 mL /min,and column temperature was 30 ℃.Results The liner range was0.395 2～4.742 4 μg,r=0.999 9,the average recovery rate was 95.60%,RSD=0.14%.Conclusion The method is simple,accurate and repeatable.It could be used in the quality control of Fuyanshu Wan.

关 键 词：高效液相色谱法 妇炎舒丸 芍药苷 

分 类 号：R286.0[医药卫生—中药学]