柴芩清肝方提取物大孔吸附树脂精制工艺研究  

Optimization of Purification Technology of Chaiqin Qinggan Formula Extract by Macroporous Resin

作　　者：付克[1] 张坤[1] 闫广利[1] 史冯琳[1] 

机构地区：[1]黑龙江中医药大学中医药研究院,哈尔滨150040

出　　处：《中国实验方剂学杂志》2011年第4期26-29,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：黑龙江省科技攻关项目资助(GC08C322)

摘　　要：目的:优化柴芩清肝方提取物大孔吸附树脂精制工艺条件。方法:以黄芩苷、甘草苷、芍药苷和总皂苷的含量为评价指标,对大孔吸附树脂的型号、吸附条件和解析条件进行了考察。结果:最佳工艺条件为:采用AB-8型大孔吸附树脂,最大上样量为4.375g生药/g树脂,上样药液质量浓度为0.75g.mL-1(生药),吸附流速为3BV.h-1,用70%乙醇以5BV.h-1的流速洗脱。结论:该工艺可用于柴芩清肝方提取物的精制。Objective：To optimize the purification technology of Chaiqin Qinggan formula extract by macro-porous resin.Method：The macroporous resin was selected and the purification process was evaluated by measuring the content of baicalin,paeoniflorin,glycyrrhizin and total saponins.Result：The AB-8 macroporous resin was select-ed with 0.75 g.mL-1 the loading concentration of crude drug and 3 BV.h-1 current velocity,70% ethanol as the e-luting reagent with the eluting velocity of 5 BV.h-1.Conclusion：The method is effective for purification of Chaiqin Qinggan formula extract.

关 键 词：大孔吸附树脂 柴芩清肝方提取物 精制工艺 

分 类 号：R283.6[医药卫生—中药学]