辛热药小复方对大鼠物质代谢及第二信使影响的动态研究  

Dynamic Study of Pungent-hot Herb Compound on Metabolism and Second Messenger

作　　者：李连珍[1] 刘小青[1] 张倩[1] 张冰[1] 

机构地区：[1]北京中医药大学中药学院,北京100029

出　　处：《中国实验方剂学杂志》2011年第4期154-156,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点基础研究发展计划项目(2007CB512605)

摘　　要：目的:动态观察辛热药附子、肉桂、干姜组成的小复方对大鼠物质代谢及第二信使相关指标的影响,以期为虚热证动物模型研究提供实验依据。方法:40只SD雄性大鼠按体重随机分成正常组和中药组,每组20只。中药组大鼠ig附子:肉桂:干姜(1:1:1)200%水煎液16 g.kg-1。在给药12,24,36,48,60 d后眼眶取血2 mL,EDTA抗凝分离血浆,全自动生化仪检测血糖(GLU)、总蛋白(TP)、总胆固醇(TG)、甘油三酯(TC)等物质代谢指标,放免法检测cAMP,cGMP第二信使相关指标。结果:与正常组比较,中药组GLU和TP含量均出现高→低→高的变化趋势,cGMP含量升高;TG,TC,cAMP变化不明显。结论:实验过程中,辛热药小复方对物质代谢和第二信使的影响随着给药时间的延长而出现波动,因此建议在虚热证动物模型研究过程中应根据实验要求选择合适的给药时间。Objective：To dynamicaly observe the effects of pungent-hot herb decoction of Radix Aconiti Lateralis Preparata,Cortex Cinnamomi Aconite and Rhizoma Zingiberis on metabolism and second messenger.Meth-od： Fourty Male SD rats were divided into two groups.The control group was given 4 mL of water;the experimental group was given 4 mL of pungent-hot decoction.About 2 mL blood samples were collected from orbit on 12th day,24th day,36th day,48th day and 60th day.Plasma GLU,TG,TC,TP levels were detected by automatic biochemical analy-zer,Plasma cAMP,cGMP levels were determinated by radio-immunity.Result： Compared with the control group,the plasma GLU and TP levels changes with the trend of high→low→high,while cGMP level was significantly higher.The other parameters were not changed significantly.Conclusion： During the experimental process,there were sig-nificant fluctuation on metabolism and second messenger.It should pay more attention on the study of Yin deficiency model and choose administration time with the experimental requirements.

关 键 词：辛热药 物质代谢 第二信使 虚热模型 动态研究 

分 类 号：R285.5[医药卫生—中药学]