单向灌流法研究紫草素的大鼠在体肠吸收  

Intestinal Absorption of Shikonin in Rats by in Situ Single Pass Perfusion Method

作　　者：苏红[1] 黄川生[1] 陈文[1] 王新春[2,1] 

机构地区：[1]石河子大学药学院,新疆石河子832002 [2]石河子大学医学院第一附属医院,新疆石河子832008

出　　处：《中国实验方剂学杂志》2011年第4期190-192,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：石河子大学科学技术研究发展计划-高层次人才科研起动资金专项(RCZX-2007-79);国家科技部课题重大基础研究前期研究专项(2004CCA02300)

摘　　要：目的:研究紫草素的大鼠在体肠吸收机制。方法:采用单向灌流模型,紫外分光光度法测定在体肠灌流紫草素的浓度变化,研究紫草素的吸收部位和吸收动力学特征。结果:紫草素在大鼠各肠段的吸收速率常数(Ka)、有效渗透系数(Papp)是结肠>空肠>十二指肠>回肠,且各肠段的Ka和Papp值均无显著性差异;灌流液中不同浓度紫草素的Ka,Papp均无统计差异。结论:紫草素在全肠道有不同程度的吸收,其中结肠吸收最好,药物浓度对紫草素的Papp和Ka值无影响,其吸收机制为被动扩散。Objective：To study the in situ intestinal absorption behaviors of shikonin in rats.Method：The in situ rat single-pass intestinal perfusion model was used and the concentrations of the perfusate were determined by UV to investagate the intestinal absorption site and mechanism.Result：The Ka and Pappof shikonin showed colon jejunum duodenum ileum in four different region of rat intestine.The Ka and Papp of every segment had no signifi-cant different from the others.The Ka and Papp of different drug concentration had no significant influence.Conclu-sion： Shikonin could be absorbed in whole intestinal segment,the best intestinal absorption site was colon.Its ab-sorbing mechanism may be related to passive diffusion.

关 键 词：紫草素 在体肠吸收 单向灌流法 

分 类 号：R285.5[医药卫生—中药学]