产氢产乙酸菌ZR-1的分离鉴定及产酸特性  

Separation and identification of a hydrogen-producing acetogens strain ZR-1 and its acetic production characters

作　　者：习彦花[1,2] 程辉彩[1] 张丽萍[1] 崔冠慧[1] 马金亮[1,3] 

机构地区：[1]河北省科学院生物研究所,河北石家庄050081 [2]河北师范大学生命科学学院,河北石家庄050016 [3]河北大学生命科学学院,河北保定071002

出　　处：《微生物学通报》2011年第2期181-186,共6页Microbiology China

基　　金：国家"十一五"科技支撑项目(No.2008BADCC4B02-8);河北省科技支撑项目(No.08225502D)

摘　　要：采用改良的亨盖特厌氧操作技术,从有机废水污泥中分离到一株耐低温高效产氢产乙酸菌ZR-1。经过对其形态学观察、生理生化特征研究及16S rRNA序列比对,初步鉴定为梭状芽胞杆菌属的乙二醇梭菌(Clostridium glycolicum)。通过单因子实验,在厌氧条件下对该菌株的培养温度、pH、最适底物、金属离子的影响等产酸条件进行了优化。结果表明该菌株最适生长温度37°C,最佳培养基初始pH值8.5,最适发酵底物丁酸盐,Mn2+对其产酸有一定的激活作用。最适培养条件下丁酸盐降解率达到12.7%,H2含量达到了28.73%。A hydrogen-producing acetogen strain ZR-1 was isolated from organic wastewater sludge by applying modified Hungate anaerobic method.According to morphological observation,physiological-biochemical characteristics and homology analysis of 16S rRNA gene sequence,the strain was identified as a genus of Clostridium glycolicum.The effects of temperature,pH,optimum substrate and metal ions on its productivity of acetic acid in anaerobic conditions were investigated and then optimized by uniform tests.The results indicated that the optimun temperature and initial pH of culture media for strain ZR-1 were 37 °C,8.5,and it's optimum substrate was butyrate.Supply of Mn2+ in culture medium can promote acetic acid production of ZR-1.At optimum cultivation conditions,the conversion rate of butyrate reached 12.7% and the content of H2 was up to 28.73%.

关 键 词：厌氧消化 产氢产乙酸菌 鉴定 生酸特性 

分 类 号：X703[环境科学与工程—环境工程]