新疆泥火山细菌群落PCR-SSCP分析  

PCR-SSCP analysis bacterial community of Mud Volcano in Xinjiang

作　　者：路盼盼[1] 李建辉[1] 张亚平[1] 

机构地区：[1]石河子大学生命科学学院,新疆石河子832003

出　　处：《微生物学通报》2011年第2期195-198,共4页Microbiology China

基　　金：国家自然科学基金项目(No.30860001)

摘　　要：采用单链构象多态性(SSCP)技术,以16S rDNA基因的V3区为靶对象,分析泥火山细菌多样性及其群落结构。通过对泥火山不同月份的不同深度土样DNA提取后,针对16S rDNA进行PCR扩增出236 bp大小片段,通过SSCP电泳对泥火山细菌进行季节性多样性分析,并对主要条带进行克隆分析。结果显示:新疆泥火山细菌不同季节多态性明显,而且这种多态性容易受生态和气候的影响;假单胞菌属是泥火山土壤优势菌群,在泥火山区土壤中广泛分布,受生态和气候环境影响较小。A technology sigle-strand conformation polymorphism(SSCP) and a target 16S rDNA gene on V3 region were used to analyze mud volcano microbial community and structure.After extracting DNA samples from different mud deepth and month,get a 236 bp fragment by PCR.The fragment was used to analyze the microorganisms seasonal diversity of mud volcano by SSCP,and some target fragments were analyzed clone.Resluts showed:the bacteria polymorphism in Xinjiang mud volcano soil is significant,which is vulnerable to ecological and climatics factors.Pseudomonas is a dominant microorganism in Xinjiang mud volcano,it distributes widely in mud volcano and was affected little by ecological and climatics.

关 键 词：泥火山 PCR-SSCP 16S RDNA 

分 类 号：Q93[生物学—微生物学]