南极抗细菌活性菌株的筛选及系统发育分析  

Screening and phylogenetic analysis of antibacterial strains isolated from antarctic

作　　者：李贺[1,2] 林学政[2] 何培青[2] 刘同军[1] 

机构地区：[1]山东轻工业学院食品与生物工程学院,山东济南250353 [2]国家海洋局第一海洋研究所国家海洋局海洋生物活性物质重点实验室,山东青岛266061

出　　处：《微生物学通报》2011年第2期264-269,共6页Microbiology China

基　　金：海洋公益性行业科研专项经费项目(No.01005032-2);国家863计划项目(No.2007AA091905);基本科研业务费专项资金项目(No.2007T11)

摘　　要：分别以大肠杆菌、枯草芽孢杆菌、金黄色葡萄球菌、青枯假单胞菌、绿脓假单胞菌和苏云金芽孢杆菌为指示菌,采用琼脂扩散法对实验室保存的580株极地细菌进行了抗菌活性菌株的筛选与活性验证,从中筛选出4株对上述指示菌株具有明显抗菌效果的活性菌株,其编号分别为97、Z11、Z18及Z19,并对其生长曲线、抗菌活性曲线和系统发育地位进行研究。结果表明,4株菌均在培养24 h后进入指数生长期,菌株97在培养48 h后达到稳定期,而菌株Z11、Z18及Z19在培养60 h后达到稳定期。抗菌活性分别在培养84、96、72和72 h时达到最高。系统发育分析表明,该4株菌分别属于伦黑墨氏菌属(Rheinheimera)、嗜冷杆菌属(Psychrobacter)、假单胞菌属(Pseudomonas)和嗜冷杆菌属(Psychrobacter)。Escherichia coli,Bacillus subtilis,Staphylococcus aureus,Pseudomonas solanacearum,Pseudomonas aeruginosa and Bacillus thuringiensis were used as indicator strains respectively,four antibacterial strains,named as 97,Z11,Z18 and Z19 were screened from 580 strains of bacteria isolated from Antarctic by the method of agar diffusion.The growth curves,antibacterial curves and phylogenetic analysis of 4 antimicrobial strains were studied.The results showed that all of the four strains reached the log phase when they were cultured 24 h,strain 97 was in the log phase when it was cultured after 48 h,and the remaining three strains Z11,Z18 and Z19,were in the stationary when they were cultured after 60 h.They had the highest antibacterial activity when it was cultured after 84,96,72 and 72 h,respectively.Phylogenetic analysis indicated that these four strains belong to genera of Rheinheimera,Psychrobacter,Pseudomonas and Psychrobacter,respectively.

关 键 词：南极细菌 抗菌活性 系统发育分析 

分 类 号：Q93[生物学—微生物学]