抗草甘膦菌种的分离鉴定与生长特性的研究  

Isolation,Identification and Growth Characters of Glyphosate-resistant Strain

作　　者：谢放[1] 田伟[1] 朱子雄[1] 

机构地区：[1]兰州交通大学化学与生物工程学院,甘肃兰州730070

出　　处：《湖南农业科学》2011年第1期82-84,88,共4页Hunan Agricultural Sciences

摘　　要：从生产草甘膦的化工厂污水处理池中分离得到草甘膦抗性菌种(Z-1)。Z-1可以在以草甘膦为唯一碳源和氮源的无机盐培养基上生长,其草甘膦耐受性达到了1 000 mg/L。并检测了LB培养基和草甘膦基础培养基中NaCl浓度和pH值对Z-1菌生长的影响。在草甘膦基础培养基中加入葡萄糖可以加快菌种降解草甘膦的速率。根据菌体特征和分子鉴定的结果,Z-1菌属于苍白杆菌属。A glyphosate-resistant strain（Z-1） was isolated from the sewage treatment pool of chemical factory which producing glyphosate.Z-1 can grow in mineral medium（MM）,which took glyphosate as exclusive carbon source and nitrogen source.The glyphosate-resistance of Z-1 reached 1 000 mg/L.Meanwhile,the influences of NaCl content and pH value in LB medium and glyphosate MM on growth of Z-1 were detected.Adding glucose to the glyphosate MM can accelerate the speed that Z-1 degrades the glyphosate.According to the characters of strain and the results of molecular identification,the Z-1 strain belongs to Ochrobactrum.

关 键 词：抗草甘膦 苍白杆菌属 分离及生长特性 

分 类 号：R282.2[医药卫生—中药学]