氯胺酮后处理及其联合缺血后处理对大鼠肝脏再灌注损伤的作用  

Effect of ketamine postconditioning and ketamine postconditioning combines with ischemic postconditioning on hepatic ischemia-reperfusion injury in rats

作　　者：方七五[1] 冷玉芳[1] 张琰[1] 

机构地区：[1]兰州大学第一医院麻醉科,兰州市730000

出　　处：《临床麻醉学杂志》2011年第1期63-65,共3页Journal of Clinical Anesthesiology

基　　金：兰州市科技发展计划项目(2009-1-52)

摘　　要：目的探讨氯胺酮后处理及其联合缺血后处理对大鼠肝脏再灌注损伤的作用。方法雄性SD大鼠24只,体重200～230g,随机均分为缺血损伤组(A组)、氯胺酮后处理组(B组)、氯胺酮后处理联合缺血后处理组(C组)、假手术组(D组)。按照Nauta介绍的方法建立缺血模型,在缺血1h后恢复灌注时,B组由尾静脉推注氯胺酮10mg/kg,C组由尾静脉推注等量氯胺酮的同时对肝脏进行缺血后处理,D组仅分离肝十二指肠韧带。再灌注4h后采集下腔静脉血测丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST);取肝组织测超氧化物歧化酶(SOD)、丙二醛(MDA)含量,检测Bcl-2和Bax的表达和通过电镜观察肝脏的损伤程度。结果 B、C组ALT、AST、MDA均低于A组、SOD高于A组(P<0.05),Bcl-2表达高于A组,Bax表达低于A组(P<0.05)。透射电镜下观察B、C组细胞损伤程度轻于A组。结论氯胺酮后处理可以减轻大鼠肝脏的再灌注损伤,但是联合缺血后处理后其保护效应并没有加强。Objective To investigate the effect of ketamine postconditioning and ketamine postconditioning combines with ischemic postconditioning on hepatic IRI in rats.Methods Twenty-four male SD rats weighing 200-230 g were divided into four groups：group A,ischemia-reperfusion injury,group B ketamine postconditioning,group C,ketamine postconditioning combines ischemic postconditioning,group D,sham-operated.According Nauta RJ＇s method to establish a model of ischemia-reperfusion injury.After 1 h ischemia,group B and group C were administrated ketamine（10 mg/kg）,but group C were administrated ischemic postconditioning at the same time.Blood samples were obtained after reperfusion 4 hours for determination of serum ALT,AST,livers were removed for determination of express of Bcl-2,Bax and MDA,SOD,also for electronic microscopic examination in each group.Results Compared with those in group A,ALT,AST,MDA and the express of Bax in group B and group C are significantly lower（P〈0.05）,SOD and the express of Bcl-2 are significantly higher（P〈0.05）.The results of electronic microscopic examination also demonstrate the injury in group B and group C are less severe than that in group A.Conclusion Ketamine postconditioning and ketamine postconditioning combines with ischemic postconditioning can reduce ischemia-reperfusion injury,but there is no difference between the two groups.

关 键 词：再灌注损伤/预防和控制 后处理 细胞保护 氯胺酮 肝脏 

分 类 号：R965[医药卫生—药理学]