乌司他丁明显减轻肠源性脓毒症大鼠氧自由基介导性肺损伤  

Ulinastatin relieves oxyradical-mediated lung injury induced by gut-origin septic status in rats

作　　者：屠伟峰[1] 韩琪[2] 孙梅[1] 郄文斌[1] 温雪飞[1] 

机构地区：[1]广州军区广州总医院全军临床麻醉中心,510010 [2]东莞市人民医院麻醉科

出　　处：《国际麻醉学与复苏杂志》2011年第1期11-15,共5页International Journal of Anesthesiology and Resuscitation

基　　金：基金项目：江苏省高校省级重点实验室开放课题项目（KJS04001）

摘　　要：目的观察乌司他丁（ulinastatin，uTI）对肠源性脓毒症大鼠肺组织及血中过氧化物歧化酶（superoxi dedismutase，SOD）及丙二醛（malondialdehyde，MDA）的影响。方法健康Wistar大鼠114只，雌雄各半，体重180g～220g，分为正常对照组（NC组，n=6）、假手术对照组（sc组，n=30）、脓毒症组（SEP组，n=30）、UTI3h治疗组（UTI。组，n=30）和UTI12h治疗组（UTIl2h组，n=18）。观察时间点包括假手术或盲肠结扎穿孔（cecal ligation and puncture，cLP）后6、12、24、48、72h。实验动物共有19个小组，在实验前按完全随机化要求进行分配。UTI，。和UTI。治疗组分别于CLP后3h和12h腹腔注射UTI5万U／kg，首次给药12h后，再次给予同等剂量UTI。分别取正常大鼠组及各组制模后6、12、24、48、72h小组心脏内血测定血浆SOD活性及MDA含量；取右肺上、中叶检测肺组织SOD活性及MDA含量；取左上肺用于湿干重（W／D）比检测。结果SC组血浆SOD活性和MDA含量在制模后各时间点与NC组比较差异无统计学意义，SEP组制模后血浆SOD活性下降、MDA含量升高，各时间点与NC组及sC组比较差异有统计学意义（P〈O．05）；UTI治疗组在制模后SOD活性下降、MDA含量上升幅度降低，与SEP组比较差异有统计学意义（P〈0．05）。SEP组自制模后肺组织MDA含量以及W／D水平升高，SOD活性降低，与sc组各时间点比较，差异有统计学意义（P〈0．05）；uTI治疗组制模后肺MDA含量和W／D水平升高、SOD降低程度较SEP组减轻，差异有统计学意义（P〈0．05）。结论肠源性脓毒症致肺组织损伤过程中，SOD和MDA是导致肺组织损伤的原因之一，而UTI可有效改善脓毒症期间血液及肺组织内MDA升高和SOD活性降低的程度，对肺组织有一定的保护作用。Objective To observe the effects of ulinastatin （UTI） on malondialdehyde （MDA） and superoxide dismutase （SOD） in plasma and lung tissue in the rats with severe gut-origin sepsis. Methods One hundred and fourteen Wistar rats were randomly divided into four groups ： normal group （n=6）, sham control group （n=30）, septic group （n=30）, UTI3 h group （n=30） and UTI12 h group （n=18）. Blood and lung samples were obtained for detecting MDA and SOD at 6, 12, 24, 48, 72 h after CLP. Gutorigin sepsis model was induced by the classic technique of cecal ligation and puncture （CLP）. The administration of UTI was injected into abdominal cavity at 3 or 12h after the end of CLP and reinjected after 12 h interval. Blood samples was taken in the normal and experimental rats at 6, 12, 24, 48,72 h after sham-operated or CLP for the determinations of MDA and SOD in plasma and lung tissue. Results In the septic group rats, MDA in plasma and lung tissue and lung W/D ratio were higher but SOD was lower significantly than that in the sham control group （P〈0.05）. As compared with SEP group, MDA in plasma and lung tissue and lung W/D ratio were decreased significantly in the UTI3 h and UTI12 h groups （P〈0.05）, and then still higher than that in sham control group. SOD in plasma and lung tissue were increased significantly in the UTI3 h and UTI12 h groups（P〈0.05 ）, and then still lower than that in sham control group. W/D in lung tissue was positively correlated with SOD in lung tissue or plasma （P〈0.01）, but negatively with MDA in lung tissue or plasma （P〈0.01）. Conclusion It is suggested that ulinastatin could improve oxyradical- mediated lung injury induced by gut-origin sepsis and block the vicious cycle of oxygen free radical cascades, and then relieve damage of remote organs.

关 键 词：乌司他丁 肠源性脓毒症状态 丙二醛 过氧化物歧化酶 

分 类 号：R459.7[医药卫生—急诊医学]