铬天青S分光光度法测定药物中的维生素C  

Determination of Vitamin C by CAS Spectrophotometric Method

作　　者：刘二东[1] 张淑芳[1] 张红[1] 

机构地区：[1]曲阜师范大学化学与化工学院,山东曲阜273165

出　　处：《山东化工》2011年第1期26-28,共3页Shandong Chemical Industry

摘　　要：利用维生素C的还原性将Fe^3＋还原为Fe^2＋，在pH值=4条件下，用铬天青S作Fe^3＋的显色剂，然后测量吸光度，从而建立了一种测定药物中维生素C的新方法。Fe^3＋-铬天青S的最大吸收在576nm，维生素C浓度在0-2mg／L范围内服从朗伯-比尔定律。其回归方程为A=0．0037C-0．0083，相关系数R为0．9989。实验证明，此法用于药物中维生素C的测定，效果良好。Vitamin C was determined by CAS spectrophotometric method using iron （Ⅲ） as the oxidizer of vitamin C. CAS was used as the colorimetric reagent for iron （Ⅲ） in the pH value = 4.0. The violet complex exhibited the absorption peak at 576nm where the Lambert - Beer law was obeyed in the Vitamin C concentration range of 0 - 2mg,/L. The linear regression equation was A = 0. 0037C -0. 0083 and its relative coefficient was 0. 9989. The proposed new method was applied to the determination of Vitamin C in medicine with satisfactory results.

关 键 词：维生素C 铬天青S 褪色光度法 

分 类 号：R927[医药卫生—药学] O657.3[理学—分析化学]