纳米细菌感染致SD大鼠前列腺结石的实验研究  

Prostatic calculus caused by nanobacteria infection in SD rats

作　　者：明爱民[1] 张新际[1] 郭君毅[1] 吴永吉[1] 王海飞[1] 沈学成[2] 宋波[2] 

机构地区：[1]解放军第三七一中心医院泌尿外科,河南省新乡市453000 [2]第三军医大学西南医院全军泌尿外科研究所

出　　处：《中华泌尿外科杂志》2011年第2期122-125,共4页Chinese Journal of Urology

摘　　要：目的建立前列腺纳米细菌（NB）感染的SD大鼠模型，探讨NB在前列腺炎以及前列腺结石形成中的致病作用。方法40只成年雄性SD大鼠随机分为对照组和模型组，每组20只。分别经尿道注人生理盐水0．2ml和NB混悬液0．2ml（浓度为1个麦氏单位）。将2组动物分别在4周、8周后处死（每次每组10只）。HE染色观察2组大鼠前列腺组织的病理改变，透射电镜和扫描电镜观察有无结石形成。取前列腺组织做NB细菌的再培养和鉴定。结果4周后模型组大鼠前列腺组织出现慢性炎症，并持续到8周后。8周后模型组7只大鼠前列腺组织中发现小结石。前列腺组织NB再培养均有NB生长。对照组动物前列腺组织未见炎症及结石，NB细菌的再培养未见NB生长。结论NB感染大鼠前列腺能造成前列腺慢性炎症，并形成结石，NB感染可能是前列腺结石形成的重要病因。Objective To reproduce an SD rat model of prostatic calculus by using nanobacteria （NB）, and explore the role of NB in contributing to prostatitis and prostatic calculus. Methods Twenty adult male SD rats were randomized to the control group and 20 to the model group. Rat pros- tate infection models were reproduced by infusing 0.2 ml （Concentration, 1 Mai unit） NB suspension transurethrally. 0.2 rnl physiological saline was infused transurethrally in the rat control group. The rats were sacrificed 4 and 8 weeks later and prostatic pathology were viewed by hematoxylin and eosin （HE） staining. Lithogenesis was observed by scanning electron microscope （SEM） or Transmission electron microscopy （TEM）. Re isolation, culture and identification of nanobacteria were also done in rat prostatic tissues. Results Chronic inflammatory changes of prostates were shown in the model group at both 4 weeks and 8 weeks after infusing NB suspension. Prostatic calculi were detected by SEM and TEM at 8 weeks in the prostates of the rat model group （7/10）. Neither chronic inflamrna tory changes nor prostatic calculus was found in the control group. NB was positive in the model group, but negative in the control group. Conclusions NB infection could cause chronic prostatitis and prostatic calculus in rats.

关 键 词：纳米细菌 前列腺炎 前列腺结石 动物模型 

分 类 号：R697.3[医药卫生—泌尿科学]