低温下混合菌降解柴油过程中脱氢酶活性的变化  

Change of Dehydrogenase Activity in Degradation of Diesel Fuel with Mixed Bacteria at Low Temperature

作　　者：邓海静[1] 刘勇波[1] 刘虹[1,2] 张兰英[1] 刘娜[1] 

机构地区：[1]吉林大学环境与资源学院,吉林长春130026 [2]吉林化工学院环境与生物工程学院,吉林吉林132022

出　　处：《化工环保》2011年第1期9-12,共4页Environmental Protection of Chemical Industry

基　　金：国家高技术研究发展计划(863计划)资助项目(2007AA06A410);吉林省科技厅资助项目(20090415)

摘　　要：研究了低温(10℃)下混合菌降解柴油过程中脱氢酶活性在不同反应时间、柴油初始质量浓度、混合菌液加入量等条件下的变化情况。实验结果表明:在柴油初始质量浓度为100 mg/L时,脱氢酶的活性始终较低;在柴油初始质量浓度为300,600,900 mg/L时,反应第1天脱氢酶的活性相差不大,随反应时间的延长,柴油初始质量浓度越高,对应的脱氢酶活性越高;在柴油初始质量浓度为450 mg/L、混合菌液加入量为5 mL的条件下,反应2天后脱氢酶的活性达最高值39.6 mg/(L·h),反应4天后柴油质量浓度为25.1 mg/L,混合菌对柴油的降解率为94.4%。The change of the dehydrogenase activity in the process of diesel fuel degradation with mixed bacteria at low temperature （ 10 ℃） under different conditions of reaction time, different initial diesel fuel mass concentration and mixed bacteria dosage was researched. The experimental results show that： When the initial diesel fuel mass concentration is 100 mg/L, the dehydrogenase activity is low all the time; When the initial diesel fuel mass concentration is 300,600,900 mg/L, the dehydrogenase activity has no remarkable change in the first day of reaction, but with the reaction time, it is positively correlated with the initial diesel fuel mass concentration; Under the conditions of initial diesel fuel mass concentration 450 mg/L and mixed bacteria dosage 5 mL, the highest dehydrogenase activity is 39.6 mg/（ L - h） after 2 days of reaction, the diesel fuel mass concentration is 25.1 mg/L and the degradation rate of diesel fuel is 94.4% after 4 days of reaction.

关 键 词：低温 混合菌 柴油 脱氢酶活性 

分 类 号：TQ116.2[化学工程—无机化工]