不同剂量X射线全身照射对小鼠脾细胞转铁蛋白受体表达的影响  

EFFECTS OF WHOLE BODY X IRRADIATION IN DIFFERENT DOSE ON EXPRESSION OF TRANSFERRIN RECEPTOR IN SPLENOCYTES OF MICE

作　　者：罗灿[1] 苏旭[1] 付士波[1] 刘建香[1] 鞠桂芝[1] 

机构地区：[1]白求恩医科大学卫生部放射生物重点实验室

出　　处：《辐射防护》1999年第4期284-288,共5页Radiation Protection

摘　　要：本文采用直接免疫荧光流式细胞术方法，检测了不同剂量Ｘ射线全身照射对小鼠脾细胞转铁蛋白受体（ＴｆＲ）表达的影响及白细胞介素－２（ＩＬ－２）活性的变化。结果表明，大剂量照射（０．５～６．０Ｇｙ）抑制ＴｆＲ的表达，且随照射剂量的增加ＴｆＲ的表达呈明显的剂量依赖性下降，４Ｇｙ及以上剂量照射明显抑制ＩＬ－２活性；小剂量（２５～２００ｍＧｙ）照射时，７５ｍＧｙ剂量照射刺激ＴｆＲ的表达并可增强ＩＬ－２活性。说明大剂量和小剂量照射对ＴｆＲ的表达产生不同的辐射效应，ＴｆＲ在辐射免疫效应产生过程中发挥作用。Using direct immunoflurescence and flow cytometric analysis,the expression of transferrin receptor(TfR) in lymphocytes in mouse spleens was examined and the activity of IL 2 was assayed after the whole body irradiation (WBI) with X rays in different doses. The findings showed that the WBI with high dose of X rays (0.5～6.0 Gy) inhibited TfR expre ssion and the expression of TfR decreased with dose increase in an obvious dose dependent manner, and that WBI in 4 Gy and more inhibited the activity of IL 2 but the WBI in 75 mGy could stimulate TfR expression and enhanced the activity of IL 2. It is demonstrated that TfR expressions play a role in induction of immune effects by ionizing radiation. (

关 键 词：脾细胞 转铁蛋白受体 X射线 剂量效应 

分 类 号：R818.02[医药卫生—放射医学] R818.85[医药卫生—临床医学]