CACPPA的^(99)Tc^m标记及其动物实验研究  

99 Tc m LABELING AND ANIMAL EXPERIME NTS OF CACPPA

作　　者：吴春英[1] 纪书仁[1] 方平[1] 周翔[1] 陈正平[1] 何拥军[1] 

机构地区：[1]江苏省原子医学研究所核医学国家重点实验室

出　　处：《同位素》1999年第2期100-104,共5页Journal of Isotopes

基　　金：江苏省自然科学基金

摘　　要：在ｐＨ８．５～９．５的水溶液中，采用氯化亚锡还原法制备９９Ｔｃｍ－ＣＡＣＰＰＡ，ＴＬＣ和ＨＰＬＣ检测其标记率和放化纯均大于９０％。小鼠体内分布表明，心肌最高摄取率为１８．１７±２．６７％ＩＤ／ｇ；小鼠血药动力学研究表明９９Ｔｃｍ－ＣＡＣＰＰＡ的血液清除快，Ｔ１／２α为１．１１ｍｉｎ，Ｔ１／２β为２０．０８ｍｉｎ，清除速率为０．４０７ｍＬ／ｍｉｎ。９９Ｔｃｍ－ＣＡＣＰＰＡ的体内外血浆蛋白结合率高，ｐＨ７．００和ｐＨ７．４０时分配系数分别为１０．９８和１１．４５；代谢干预研究结果表明，葡萄糖胰岛素组心肌摄取升高，与对照组相比，差异具有显著性意义。Tc m CACPPA is prepared by the reduct ion of stannous chloride with Na 99 Tc mO - 4 in aqueous soluti on a t pH8.5￣9.5,the labeling yield and radiochemical purity are over 90% determined by TLC and HPLC. Biodistribution of 99 Tc m CACPPA in mice demonstra tes that the highest myocardial uptake of 99 Tc m CACPPA is 18.17±2. 67% ID/g. When blood disappearance of 99 Tc m CACPPA is analyzed with a biexponential model, an initial half time of 1.11 min and a late half tim e of 20.08 min are obtained. 99 Tc m CACPPA exhibite high binding to HSA in vitro and in vivo, partition coefficients are 10.98 and 11.45 at pH7.00 a nd pH7.40 respectively. The study of metabolism intervention showes that myocard ial uptake of 99 Tc m CACPPA in the group of glucose insulin is high er than that of normal, which has significant difference after t test.

关 键 词：CACPPA 锝99标记 动物实验 

分 类 号：R817.42[医药卫生—影像医学与核医学]