六味地黄丸含药血清对HK-2细胞TGF-β/Smad信号通路的影响  

Effect of drug-containing serum of Liuwei Dihuang pills on TGF-β/Smad signaling pathway in HK-2 cells

作　　者：郝艳鹏[1] 张悦[1] 刘煜敏[1] 陆敏[1] 王飞[1] 陆海英[2] 

机构地区：[1]上海中医药大学科技实验中心,上海201203 [2]上海中医药大学护理学院,上海201203

出　　处：《中国病理生理杂志》2011年第1期149-152,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30371834);上海市教育委员会重点学科建设项目(第5期;No.J50301);上海高校创新团队建设项目

摘　　要：目的:观察六味地黄丸含药血清对HK-2细胞增殖及其Smad通路信号影响。方法:MTT法检测大鼠5%、10%、20%空白血清与5%、10%、20%含药血清对HK-2细胞增殖的影响,并采用蛋白印迹法检测10%含药血清对HK-2细胞Smad通路信号分子蛋白表达的影响。结果:六味地黄丸含药血清可以促进HK-2细胞增殖;在HK-2细胞中,TGF-β1可明显促进Smad2蛋白的磷酸化,而10%六味地黄丸含药血清具有明显的抑制效应。10%六味地黄丸含药血清亦可促进SnoN蛋白表达,明显高于对照组和空白大鼠血清组。结论:六味地黄丸含药血清能够抑制TGF-β1诱导的HK-2细胞Smad2蛋白的磷酸化,促进TGF-β/Smad通路的负性调控因子SnoN蛋白的表达。AIM：To investigate the effect of the drug-containing serum of Liuwei Dihuang pills on the TGF-β1/Smad signaling pathway in HK-2 cells.METHODS：The proliferation of HK-2 cell was detected by MTT method.Western blotting analysis was used to investigate the effect of the drug-containing serum of Liuwei Dihuang pills on the protein expression of the molecules of Smad signal transduction pathway.RESULTS：The drug-containing serum of Liuwei Dihuang pills promoted the proliferation of HK-2 cells.The level of Smad2 phosphorylation in HK-2 cells treated with 10% drug-containing serum of Liuwei Dihuang pills was significantly lower than that in the cells treated with TGF-β1.Furthermore,SnoN,a negative factor in Smad signaling pathway,was up-regulated in HK-2 cells treated with 10% drug-containing serum.CONCLUSION：Drug-containing serum of Liuwei Dihuang pills inhibits TGF-β1/Smad signaling pathway,including reducing Smad2 phosphorylation and promoting SnoN protein expression.

关 键 词：六味地黄丸 肾小管上皮细胞 信号转导 蛋白表达 

分 类 号：R285[医药卫生—中药学]