妊娠期高血压疾病患者子宫蜕膜NK细胞表型分析  

Phenotype of decidual NK cells in women with hypertensive disorder complicating pregnancy

作　　者：周娟[1] 肖小敏[1] 

机构地区：[1]暨南大学附属第一医院妇产科,广东广州510632

出　　处：《中国病理生理杂志》2011年第1期183-186,共4页Chinese Journal of Pathophysiology

摘　　要：目的:观察妊娠期高血压疾病患者子宫蜕膜自然杀伤细胞(dNK细胞)的表型。方法:选取2008年8月至2009年3月在广州市暨南大学附属第一医院妇产科因妊娠期高血压疾病行剖宫产的单胎妊娠孕妇20例作为妊娠期高血压组,随机选取同期在此院因社会心理因素行选择性剖宫产的正常单胎妊娠孕妇15例作为正常对照组。收集孕晚期子宫蜕膜组织,机械研磨加梯度离心法提取蜕膜内单核细胞,流式细胞技术(FCM)筛选出dNK细胞,并检测dNK细胞表面CD56及CD16的表达情况。结果:(1)妊娠期高血压组与正常对照组CD56brightCD16-CD3-dNK细胞的数量均多于CD56dimCD16+CD3-dNK细胞,且差异显著(均P<0.01)。(2)妊娠期高血压组与正常对照组CD56brightCD16-CD3-dNK细胞所占比例无显著差异(P>0.05),CD56dimCD16+CD3-dNK细胞所占比例亦无显著差异(P>0.05)。(3)妊娠期高血压组与正常对照组dNK细胞表面CD16分子的表达率并无显著差异(P>0.05)。结论:妊娠期高血压疾病患者与正常孕妇孕晚期子宫蜕膜内dNK细胞表型无明显改变,均以CD56brightCD16-CD3-亚型为主。AIM：To investigate the phenotype of uterine decidual natural killer cells（dNK cells） in women with hypertensive disorder complicating pregnancy（HDCP）.METHODS：All the study subjects were collected from Department of Obstetrics and Gynecology,The First Affiliated Hospital of Jinan University,Guangzhou,China.Twenty cases of singleton pregnancy who underwent caesarean section because of HDCP were selected as HDCP group,and 15 cases of singleton pregnancy received selective caesarean section because of social-psychological concerns were also randomly selected as normal control group.The decidual tissues were sampled immediately after caesarean section.The mononuclear cells were extracted from the tissues by means of mechanic grinding and gradient centrifugation.The technique of flow cytometry was used for dNK cell sorting and the expression of CD56 and CD16 on the surface of cells was also examined.RESULTS：In HDCP group and normal control group,the proportions of CD56brightCD16-CD3-dNK cells were significantly higher than those of CD56dimCD16＋CD3-dNK cells（P〈0.01）.Neither the CD56brightCD16-CD3-subset nor the CD56dimCD16＋CD3-subset had statistical difference between HDCP group and normal control group.No significant difference of CD16 expression on the surface of dNK cells between HDCP group and normal control group was observed（P〉0.05）.CONCLUSION：The phenotypes of dNK cells from the women with HDCP and from healthy pregnant women are both dominated by CD56brightCD16-CD3-subset,without significant difference.

关 键 词：子宫蜕膜 自然杀伤细胞 妊娠高血压 母-胎界面 细胞表型 

分 类 号：R714.2[医药卫生—妇产科学] R363.2[医药卫生—临床医学]