胰岛、肝细胞水平胰岛素抵抗与系统胰岛素抵抗关系的研究  

The investigation of relation between the insulin resistance on cell level in liver or pancreas and systematical insulin resistance

作　　者：向静[1] 阿布力克木.吐尔地 王宁[2] 

机构地区：[1]新疆医科大学第一附属医院内分泌科,新疆乌鲁木齐830011 [2]新疆医科大学第一附属医院干内一科,新疆乌鲁木齐830011

出　　处：《新疆医科大学学报》2011年第1期1-4,共4页Journal of Xinjiang Medical University

基　　金：国家自然科学基金(30801019)

摘　　要：目的通过观察酪氨酸磷酸化的胰岛素受体底物(P-Ikbα)在胰岛素抵抗小鼠肝脏、胰腺的表达随时间变化的差异性,了解细胞水平胰岛素抵抗与系统胰岛素抵抗的关系。方法 C57小鼠随机分为高脂组、胰岛素兴奋组和对照组,均尾静脉监测血糖、体重及胰岛素水平变化。取肝脏、胰腺应用Western Blot方法检测P-Ikbα的表达。结果与对照组相比,高脂组和胰岛素兴奋组肝脏、胰腺P-Ikbα蛋白表达随时间延长而减少。血清胰岛素水平在造模第7天升高,而肝脏的IRS-1酪氨酸磷酸化在第14天末低于正常对照组,胰腺在第21天末低于正常组。结论肝脏IRS-1酪氨酸磷酸化水平的降低早于胰腺;血清胰岛素升高发生时间早于细胞水平胰岛素抵抗发生时间。Objective To study the expressions of P-Ikbαin liver and pancreas with insulin resistance（IR） mouse in dirrerent time,to investigate the relationship between the IR on cell level and systematical insulin resistance.Methods One hundered and fifty C57 mice were randomized into 3 groups.The normal control（NC） group with the normal diet and the model group fed with highfat diet for 12 weeks.The levels of blood insulin,weight and blood glucose were determined.The expression of P-Ikbα in liver and pancreas were examined by Western blot.The data were analyzed by statistics software SPSS 17.0.Results The expression of P-Ikbα in tissues were markedly decreased compared with NC group（P0.05）.The level of insulin in blood was increased in 7th day.The expression of P-Ikbα in liver and pancreas were decreased at the 14th day and the 21st day.Conclusion The expression of P-Ikbα in liver are earlier than pancreas.The occurrence of insulin resistance in systematical are earlier than cell level.

关 键 词：胰岛素抵抗 P-Ikbα 小鼠 

分 类 号：R587[医药卫生—内分泌]