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作 者:陈志宏[1,2] 谷继承[2] 李晓芳[2] 贠旭疆[2] 高洪文[1]
机构地区:[1]中国农业科学研究院北京畜牧兽医研究所,北京100193 [2]全国畜牧总站,北京100125
出 处:《草业学报》2011年第1期148-154,共7页Acta Prataculturae Sinica
基 金:现代化产业体系专项资金;十一五科技支撑(2008BADB3B01)资助
摘 要:采用硅胶干燥法将塔落岩黄芪种子含水量由原来的7.900%降至4.645%,3.460%,2.921%和1.829%,用铝箔袋密封后在-4℃、4℃、室温和45℃条件下贮存12个月后测定各项生理生化指标。结果表明,种子含水量在2.921%~4.645%内可以提高塔落岩黄芪种子的耐贮性,增加塔落岩黄芪种子膜透性,增加SOD、POD、CAT活性分别至4.74U/mg,0.233U/mg,2.91U/(min.g),降低MDA含量至59.72nmol/L,3.460%为塔落岩黄芪种子超干贮存的适宜含水量。室温适度超干一定程度上可以起到低温未超干的保存效果,因此认为适度超干可以作为塔落岩黄芪种质资源保护的有效措施。The effect of ultra-dry treatment on seed storage of Hedysarum laeve was researched.The seed moisture contents were reduced from 7.900% to 1.829%,2.921%,3.460%,or to 4.645% by silica gel,and were then stored at-4℃,at 4℃,at room temperature,or at 45℃.They were tested for physiological and chemical indexes after 12 months.Appropriate ultra-dry treatment enhanced storage effectiveness,increased membrane permeable and activity of SOD,POD,and CAT,but reduced the MDA content of H.laeve seeds.The optimal moisture content for ultra-dry storage was 3.460%.Ultra-drying at room temperature resulted in the same storage effects as treatment at low temperature.Appropriate ultra-dry treatment was therefore an effective conservation measure for H.laeve germplasm resources.
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