体外批次培养法研究莫能菌素对亚油酸氢化及反式11-油酸合成的影响  

A Batch Culture Method for Evaluating the Effects of Monensin on Hydrogenation of Linoleic Acid and Synthesis of Trans11-oleinic Acid

作　　者：刘立成[1,2] 金龙[1] 宋伟红[2] 刘大森[1] 

机构地区：[1]东北农业大学动物科学技术学院,哈尔滨150030 [2]黑龙江省农垦科学院畜牧兽医研究所,哈尔滨150038

出　　处：《动物营养学报》2011年第2期343-348,共6页CHINESE JOURNAL OF ANIMAL NUTRITION

摘　　要：采用体外批次培养技术,在以亚油酸(C18∶2)为脂肪酸源的培养液中添加不同水平的莫能菌素,研究其对C18∶2氢化及反式11-油酸(t11-C18∶1)合成的影响。试验采用单因子试验设计,以微晶纤维素为培养底物,莫能菌素以其占底物的质量浓度添加,对照组、M1组、M2组、M3组和M4组莫能菌素添加水平分别为0、10、20、30和40mg/kg。于培养后2、4、8、16和24h5个时间点采集样品,每个时间点4个重复,分别测定各时间点样品中硬脂酸(C18∶0)、t11-C18∶1和顺式9,顺式12-亚油酸(c9,c12-C18∶2)含量。结果表明:添加莫能菌素能显著降低培养液中C18∶0含量(P<0.05),显著提高t11-C18∶1和c9,c12-C18∶2含量(P<0.05);且莫能菌素浓度高于10mg/kg时培养液中t11-C18∶1含量随着莫能菌素浓度的增加逐渐升高。结果提示,体外培养液中添加莫能菌素能提高t11-C18∶1含量,且莫能菌素浓度超过10mg/kg时t11-C18∶1合成随剂量增加而加强。The study was conducted to evaluate the effects of different supplemental levels of monensin on the hydrogenation of linoleic acid（C18∶2） and the synthesis of trans11-oleinic acid（t11-C18∶1） in culture medium,which contained C18∶2 as the source of fatty acids,by a in vitro batch culture method.Single-factor experimental design was used in the experiment.Avicel was used as the substrate.Monensin was added by its mass concentration to the substrate and the levels were 0（control group）,10（group M1）,20（group M2）,30（group M3）,and 40 mg/kg（group M4）,respectively.Samples were collected after 2,4,8,16 and 24 h of incubation,four replicates at each time point.Contents of stearic acid（C18∶0）,t11-C18∶1 and cis9,cis12-linoleic acid（c9,c12-C18∶2） were determined.The results showed as follows： the content of C18∶0 in culture medium was reduced significantly（P0.05）,meanwhile,t11-C18∶1 and c9,c12-C18∶2 contents were significantly increased（P0.05） by the supplemention of monensin in vitro;content of t11-C18∶1 was increased as the supplemental level of monensin increased when it was higher than 10 mg/kg.The results indicate that the supplemention of monensin in culture medium can increase the t11-C18∶1 content,and the synthsis of t11-C18∶1 was enhanced as the increasing of monensin level after the level is higher than 10 mg/kg.

关 键 词：莫能菌素 亚油酸 反式11油酸 体外批次培养法 

分 类 号：S816.7[农业科学—饲料科学]