华蟾毒精对胃腺癌细胞MGC-803作用的研究  

Effect of Cinobufagin on Human Gastric Cancer MGC-803 Cell

作　　者：王小亮[1] 宋颖[2] 田秀丽[1] 何忠梅[3] 王珊[1] 张瑾[1] 邓旭明[1] 宋宇[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]长春市宽城区医院内科,吉林长春130051 [3]吉林农业大学中药材学院,吉林长春130117

出　　处：《中国畜牧兽医》2011年第2期48-51,共4页China Animal Husbandry & Veterinary Medicine

摘　　要：研究华蟾毒精(cinobufagin,CBG)体外对人胃腺癌细胞MGC-803的抑制作用,并初步探讨其抗肿瘤的作用机制。MTT法测定CBG对胃腺癌MGC-803细胞的生长抑制情况;采用光镜观察CBG对人胃腺癌细胞MGC-803形态的影响;流式细胞术检测CBG对MGC-803细胞的抑制作用、细胞周期及细胞早期凋亡的影响。一定浓度范围内CBG对MGC-803细胞的增殖有剂量、时间依赖性抑制作用;光镜下可见细胞形态明显发生变化,细胞变圆,分泌颗粒增多,细胞折光性下降,大部分细胞脱壁;流式细胞仪检测MGC-803细胞,G1期细胞减少,大量细胞停滞在G2/M期,凋亡细胞增多。CBG对MGC-803细胞的抑制作用与诱导其细胞阻滞及凋亡密切相关。To detect the inhibition of cinobufagin（CBG） on human gastric cancer MGC-803 cells and study its anti-tumor mechanism.The inhibition of cell growth of CBG was measured by MTT assay.Morphological changes were observed by optical microscopy.Apoptosis and cell cycle distribution of MGC-803 cells were determined by flow cytometry.Apoptosis of MGC-803 cell was detected by Annexin V/PI method.Cinobufagin inhibited the growth of MGC-803 cells.MGC-803 cells treated with cinobufagin showed typical characteristics of apoptosis.The cells became round,cell detachment,membrane blebbing,and cell shrinkage with a condensed cytoplasm.A net increase in the percentage of cells at G2/M phase was encountered,and this phase was maintained throughout the treatment time.Annexin V/PI flow cytometry analysis showed that CBG can cause MGC-803 cells in early apoptosis.CBG inhibited the growth of gastric cancer MGC-803 cells,induced morphological changes,made cells arrest at G2/M phase and induced apoptosis of MGC-803.The mechanism may be related to the induction of apoptosis and cell cycle arrest.

关 键 词：华蟾毒精 抗肿瘤 凋亡 周期阻滞 

分 类 号：R979.1[医药卫生—药品]