鸡小肠上皮细胞的分离培养与鉴定  

Culture of Chicken Intestine Epithelial Cells in vitro and its Characterization

作　　者：李艳[1] 彭春燕[1] 梁榕旺 赵国琦[1] 金晓君[1] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]广东博罗县畜牧兽医局,广东博罗516100

出　　处：《中国畜牧兽医》2011年第2期68-72,共5页China Animal Husbandry & Veterinary Medicine

摘　　要：选择组织块法分离培养鸡小肠上皮细胞(intestinal epithelial cells,IEC),采用机械刮除法和相差消化-相差贴壁法纯化细胞,0.05%的Trypsin-EDTA对获得的IEC进行消化传代,免疫细胞化学法鉴定IEC。结果表明,组织块培养法可分离出活性较强的IEC,并获得纯化的上皮细胞;形态学和免疫细胞化学法检测显示获得的细胞表面抗原呈阳性,鉴定为IEC;纯化的IEC可在体外稳定传代。In this study,we selected tissue culture method to isolation chicken intestinal epithelial cells（IEC）,using curettage,phase contrast digest and adherence methods to purify cells,with 0.05% Trypsin to digest and passage,immunocytochemical method to identify intestinal epithelial cells.The results showed that hadro-activity intestinal epithelial cells could be dissociated by tissue culture method,and obtain purified intestine epithelium.Morphology and immunocytochemical method showed that intestinal epithelial cells surface antigen demonstrate masculine,and purified intestine epithelium could be passaged in vitro.

关 键 词：鸡胚 小肠上皮细胞 体外培养 鉴定 

分 类 号：S811.2[农业科学—畜牧学]