运用SYBR Green Ⅰ熔解曲线法检测饲料中牛、羊源性成分的研究  

Research of SYBR Green Ⅰ Melting Curve Detection for Derived Materials in Feed in Bovine and Sheep

作　　者：班付国 刘宏伟 高延玲 马俊 

机构地区：[1]河南省饲料产品质量监督检验站,河南郑州450000

出　　处：《中国畜牧兽医》2011年第2期128-131,共4页China Animal Husbandry & Veterinary Medicine

摘　　要：试验旨在建立SYBR GreenⅠ熔解曲线法对饲料中牛、羊源成分进行检测,以SYBR GreenⅠ为荧光染料,通过优化PCR反应体系,提高了检测灵敏度。在随机抽检的145批样品中,与国标法(GB/T20190-2006)相比,SYBR GreenⅠ熔解曲线法的准确性达到了100%,此方法可作为饲料中牛羊源成分检测的一种方法。DNA melting curve analysis of SYBR Green Ⅰ was to be established as a method for the detection of dovine and sheep derived materials in feed. SYBR Green Ⅰ was used as fluorescent. The sensitivity was improved by optimizing the reaction system. Comparing with the method of GB/T20190 2006, the accuracy of the method of DNA melting curve analysis was 100%. The method of DNA melting curve analysis could be used for the dectetion of bovine and sheep derived materials in feed.

关 键 词：牛羊源成分 SYBR Green Ⅰ 荧光染料 熔解曲线 

分 类 号：S786[农业科学—林学]