致病性水貂肠炎病毒MEV-ZJ1株的分离与鉴定  

Isolation and Identification of Pathogenic Mink Enteritis Virus MEV-ZJ1 Strain

作　　者：谭斌[1] 陈微晶[2] 武华[1,2] 

机构地区：[1]中国农业科学院特产研究所人兽共患病研究室,吉林吉林132109 [2]北京必威安泰生物科技有限公司,北京100176

出　　处：《中国畜牧兽医》2011年第2期149-152,共4页China Animal Husbandry & Veterinary Medicine

基　　金："十一五"国家科技支撑计划重点项目资助(2009BADB4B00)

摘　　要：自临床疑似水貂病毒性肠炎发病貂的粪便中分离出1株病毒,经病毒形态观察、理化特性检测、血清学、聚合酶链式反应(PCR)和动物试验鉴定表明,该分离毒为水貂肠炎病毒(mink enteritis virus,MEV),命名为MEV-ZJ1株。用分离毒接种水貂,试验动物均表现出典型水貂病毒性肠炎临床症状。研究表明,MEV-ZJ1分离株对水貂具有致病性,是1株强毒,为进一步开展该病毒流行病学、致病机理、疫苗免疫与诊断检测的研究奠定了基础。A virus strain was isolated from feces of a mink suspected MEV infection.It was demonstrated to be MEV by morphology,physical and chemical properties,serum test,PCR and animal test,and it was named MEV-ZJ1.Minks were artificially infected by MEV-ZJ1 developed obvious symptoms of enteritis post-inoculation.The MEV-ZJ1 strain was virulent for minks.The successful isolation of MEV-ZJ1 provided a basis for further search on epidemiology,pathogenesis,vaccination and diagnostic.

关 键 词：水貂肠炎病毒 强毒 分离与鉴定 

分 类 号：S852.65[农业科学—基础兽医学]