3,4-二氯苯丙烯酰另丁胺对分离的神经细胞内游离钙离子的影响  

Effects of 3,4dichlorophenylpropenoylsec.butylamine on the intracellular free calcium in rat isolated brain cells

作　　者：王迪[1,2,3] 陶成[1,2,3] 李晓东 

机构地区：[1]锦州医学院药理教研室 [2]北京医科大学基础医学院药理学系 [3]锦州市中心医院麻醉科

出　　处：《中国药学杂志》1999年第6期372-374,共3页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金

摘　　要：目的：研究新的桂皮酰胺类化合物３，４二氯苯丙烯酰另丁胺（简称ＡＥＤ８８０１）对分离的神经细胞内游离钙离子的影响。方法：采用Ｃａ２＋指示剂Ｆｕｒａ２作为细胞内钙离子的荧光探针，利用ＲＦ－５０００型双波长荧光分光光度计检测分离的神经细胞内游离钙离子及其变化，并观测ＡＥＤ８８０１对静息态细胞内钙离子、高Ｋ＋去极化状态及ＮＭＤＡ刺激情况下的细胞内钙离子变化的影响。结果：在静息状态下，细胞内钙离子浓度［Ｃａ２＋］ｉ为（２０６．５±１１．２）μｍｏｌ·Ｌ－１，高Ｋ＋去极化及ＮＭＤＡ刺激后［Ｃａ２＋］ｉ均增加。ＡＥＤ８８０１０和１００μｍｏｌ·Ｌ－１可以轻度降低静息态［Ｃａ２＋］ｉ，但无统计学意义（Ｐ＞０．０５），对高Ｋ＋去极化引起的［Ｃａ２＋］ｉ增加无明显的影响（Ｐ＞０．０５），而可降低由于ＮＭＤＡ引起的［Ｃａ２＋］ｉ增加，以１０μｍｏｌ·Ｌ－１和１００μｍｏｌ·Ｌ－１作用明显，与对照组比较Ｐ＜０．０５和Ｐ＜０．０１。结论：ＡＥＤ８８０１对电压依赖性的离子通道无明显的影响。OBJECTIVE: To investigate the influence of 3,4dichlorophenylsec.butylamine (AED8801),a new derivative of cinnamamides on the intracellular free calcium in rat isolated brain cells. METHOD: Using Ca2+sensitive fluorescent indicator,Furo2 and a fluorescence detector (Shimadzu RF5000,Japan),intracellular free Ca2+(]i) was measured.The influence of AED8801 on the i resting status,depolarization induced by KCl and the addition of NmethylDaspartate (NMDA) were observed. RESULTS: The average intracellular free Ca2+ concentration in resting status was 206.5+11.2 molL-1,depolarization induced by KCl and NMDA can increase i respectively.3, 4dichlorophenylpropenoylsec.butylamine 1,10 and 100molL-1 decreased the resting status i,but no statistic significance (P>0.05) inhibited the NMDAinduced i elevation (P<0.05,P<0.01),and did not decrease the high extracellular K+induced 2+]i elevation (P>0.05). CONCLUSION: AED8801 can inhibit the Ca2+ channel of receptordependent,but can not inhibit the Ca2+ channel of voltagedependent.

关 键 词：神经细胞 钙离子 脑缺血 AED8801 

分 类 号：R972[医药卫生—药品] R743.31[医药卫生—药学]