亚甲蓝光敏法对人血浆中病毒的灭活研究  

STUDY ON INACTIVATION OF VIRUS IN HUMAN PLASMA BYMETHYLENE BLUE PHOTODYNAMIC METHOD

作　　者：张文福[1] 袁庆霞[1] 王宏霞[1] 李成文[1] 刘育京[1] 

机构地区：[1]军事医学科学院微生物流行病研究所

出　　处：《中国消毒学杂志》1999年第2期65-68,共4页Chinese Journal of Disinfection

摘　　要：向人血浆中加入１μｍｏｌ／Ｌ亚甲蓝，以１００００ｌｘ强度的溴钨灯光照射５ｍｉｎ，可灭活１０６５ＴＣＩＤ５０的水泡性口炎病毒与１０７４１ＴＣＩＤ５０的辛德毕斯病毒；作用２ｍｉｎ可灭活１０５０９ＴＣＩＤ５０的人免疫缺陷１型病毒。但作用６０ｍｉｎ仍不能破坏ＨＢｓＡｇ抗原性。Experimental observation on efficacy of combination of methylene blue and light irratiation (methylene blue photodynamic method) in inactivating the viruses in human plasma in vitro was carried out in laboratory. Adding methylene blue 1 mol/L to human plasma and irradiation with the light from bromine tungsten lamp at 10 000 lx intensity for 5 min could inactivate 1065TCID50of vesicular stomatitis virus (VSV) and 10741TCID50of Sindbis virus (SV). Exposure for 2 min could inactivate 105.09TCID50of HIV-1, while the inactivating efficacy of either methylene blue or light alone was very low. However, this combination with a 60 min exposure time had no obvious destructive effect on HBsAg antigenicity.

关 键 词：亚甲蓝光敏法 血浆消毒 输血疗法 

分 类 号：R457.14[医药卫生—治疗学] R187[医药卫生—临床医学]