亚甲兰光化学法对红细胞膜损伤的研究  

The Damaging Effect of Methylene Blue Photochemical Technique on RBC Membranes

作　　者：李秀娟 陈静静 黄革玲 

机构地区：[1]福建省厦门市妇幼保健院检验科,361000

出　　处：《国际检验医学杂志》2011年第2期181-182,184,共3页International Journal of Laboratory Medicine

摘　　要：目的分析亚甲蓝(MB)对红细胞膜的损伤作用,探讨亚甲蓝光化学法(MBP)在红细胞制品病毒灭活方面的可行性。方法以MBP处理红细胞悬液,光照度为40 000 LUX,MB浓度为5.0μmol/L,在光照0、20、40、60 min后的不同时间点测定红细胞膜Na+-K+-ATP酶和AchE活性,并观察红细胞形态的变化及膜通透性的改变。结果在红细胞制品灭活方面,MBP还有缺陷,对红细胞膜的损伤较明显。随着照射时间的延长,红细胞的边缘出现了不规则、粘连,附着物也逐渐增多。红细胞的溶血度显著增大(P<0.05),几乎呈线性。在照射过程中,膜Na+-K+-ATP酶无明显下降(P>0.05)。膜AchE在40 min后下降速率增大,与对照组比,下降了大约65%(P<0.05)。在照射20 min后渗透脆性也渐出现明显升高(P<0.05)。结论 MBP对红细胞膜有损伤作用。Objective To evaluate the effect of 5.0μmol/L methylene blue concentration that is subjected to irradiation intensity of 40 000 LUX within 0,20,40 and 60 minutes on the erythrocyte suspension using photochemical treatment method;and to observe the effects on structure and biological function of erythrocyte treated with methylene blue,and to determine the feasibility of apply ing methylene Blue to RBC suspension. Methods Methylene blue concentration of 5.0 μmol/L was added to el-ythrocyte suspension and was subjected to 40 000 LUX illuminance within 0,20,40,and 60 minutes. Erythrocyte membranes were then extracted to detect Na ＋ K＋-ATP activity,acetylcholinesterase（AchE） activity and to observe the variations of erythrocytesr shape and permeability. Results Methylene blue caused serious damage to erythrocyte membrane a suggested by the result of photochemical treatment made on erythrocyte suspension. As the irradiation time was prolonged, the structure of erythrocyte showed irregularities and accre- tions,as well as the increase of lesions. Hematocytolysis degree increased notably（P〈0.05）, nearly approaching linear. Na＋-K＋ ATP enzyme did not slow down notably（P〉 0.05） as the irradiation time was prolonged. AchE decreased significantly by 65 % （P〈0.05） after 40 minutes of irradiation time. Osmotic fragility dramatically increased after irradiating for 20 minutes（P〈0.05）, then significantly increased with longer irradiation time. Conclusion The damages to erythroeyte membranes induced by MB photo- chemical method were existed.

关 键 词：亚甲蓝 红细胞膜 研究 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]