某地区淋球菌流行株耐药性及质粒谱型研究  

The Research on the Antibiotic Resistance and Plasmid Profiles of Neosseria Gonorrhoeae in Liaocheng

作　　者：初瑞雪[1] 孟卫东[1] 

机构地区：[1]山东省聊城市人民医院,252000

出　　处：《国际检验医学杂志》2011年第2期203-204,206,共3页International Journal of Laboratory Medicine

摘　　要：目的检测该地区淋病奈瑟菌(NG)临床分离株的耐药性和质粒谱型,为临床合理用药和连续监测NG耐药性动态改变提供参考。方法从本院门诊淋病患者分泌物中分离NG菌株,检测其对常用治疗药物的最小抑菌浓度、是否产青霉素酶,提取并分析质粒谱型。结果分离获得52株NG,其中产青霉素酶17株(32.69%),四环素耐药15株(28.85%),环丙沙星耐药51株(98.08%),大观霉素耐药1株(1.92%),未发现头孢曲松钠耐药株;45株(86.54%)检出4种质粒,6种质粒谱型。结论大观霉素和头孢曲松钠可作为该地区淋病治疗首选用药;该地区NG耐药性以质粒介导耐药为主。了解质粒谱型变化有助于对流行株进行跟踪和从分子水平上为流行病学研究提供信息。Objective To analyse the resistance of Neisseria gonorrhoeaes（NG） to commonly used antibiotics and related plas- raids profiles,to provide reference for rational drug use and continous monitoring of dynamic changes of their drug resistance. Meth- ods NG strains were isolated from clinical patienst with gonorrhoeaes. Minimal inhibitory concentration,penicillinase and plasimids profiles were detectd. Results 52 strains of NG were coilected,among which there were 17 （32.69%） produding penicillinase, 15 （28.85 % ） resistant to tetracycline, 51 （98.08 %） resistant to ciprofloxacin and 1 （1.92 % ） resistant to were resistance to spectino- mycin and not any strain resistance to ceftriaxone. 4 kinds of plasmids and 6 of plasmids profiles were detectd in 45 （86. 54%） strains. Conclusion Ceftriaxone and spectinomycin could be the first choice for the therapy of gonorrhoeaes. Most of drug resistance of NG in Liaocheng were mediated by plasmid. Plasmid profiles detection is helpful for the research of prevailing strains and epidemiologic study.

关 键 词：抗菌药 抗药性 细菌 微生物敏感性试验 质粒 质粒图谱 奈瑟氏淋球菌 

分 类 号：R446.5[医药卫生—诊断学]