抗孕唑对假孕大鼠的溶黄体作用  

LUTEOLYTIC EFFECT OF DL111IT IN PSEUDOPREGNANT RATS

作　　者：杨波[1,2] 曹霖[1,2] 方瑞英[1,2] 顾芝萍[1,2] 

机构地区：[1]中国科学院上海药物研究所 [2]浙江大学药学院药理教研室

出　　处：《药学学报》1999年第5期321-324,共4页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金;中国科学院上海药物研究所新药研究国家重点实验室资助

摘　　要：目的：测定抗孕唑对假孕大鼠黄体和蜕膜的作用，以探索抗孕唑抗早孕作用的机制。方法：用放射免疫法和紫外分光光度计等方法。结果：抗孕唑可缩短大鼠假孕期４８ｄ；降低卵巢重量，蛋白含量和血清孕酮浓度；给药５ｄ后，３βＨＳＤ活性显著低于对照组；２０αＨＳＤ的活性则在给药１ｄ后即非常显著上升；子宫ＰＧＦ２α含量也显著升高，而给药５ｄ后，子宫和血清中的ＰＧＦ２α分别为对照组的３２倍和２２倍。结论：抗孕唑的溶黄体作用是由于降低假孕大鼠血清孕酮水平和增加ＰＧＦ２α含量所致，似乎与胚胎坏死无关。AIM: To observe the luteolytic effects of DL111IT in pseudopregnant rats. METHODS: Radioimmunoassay was used to estimate serum progesterone concentration and PGF2 content in uterus and serum; the activities of 3HSD and 20HSD in ovaries were assayed spectrophotometrically. RESULTS: DL111IT was shown to shorten normal pseudopregnant period of rats by 48 days and decrease ovarian fresh weight, protein content and serum progesterone level dramatically. After im DL111IT for 5 d, activities of 3HSD of pseudopregnant rats decreased 538%, while that of 20HSD incresed significantly after only one injection of DL111IT (control: 1004 mumg-1 protein; treatment: 6918 mumg-1 protein); PGF2 concentration in uterus and serum of pseudopregnant rats increased 401% and 365%, respectively. CONCLUSION: DL111IT induced luteolysis in pseudopregnant rats.

关 键 词：抗孕唑 溶黄体 卵巢黄体 早孕 大鼠 

分 类 号：R984[医药卫生—药品] R169.42[医药卫生—药学]