异丹叶大黄素和白藜芦醇对小鼠腹腔巨噬细胞白细胞介素6mRNA表达的影响  

EFFECTS OF ISORHAPOTIGENIN AND RESVERATROL ON INTERLEUKIN 6 mRNA EXPRESSION FROM STIMULATED PERITONEAL MACROPHAGES OF MICE

作　　者：钟淼[1,2] 程桂芳 赖春宁[1,2] 洪海燕 朱秀媛[1,2] 

机构地区：[1]中国医学科学院中国协和医科大学药物研究所 [2]军事医学科学院三所

出　　处：《药学学报》1999年第5期329-332,共4页Acta Pharmaceutica Sinica

摘　　要：目的：观察异丹叶大黄素和白藜芦醇对分别由炎性三肽（ｆＭＬＰ）和钙离子载体Ａ２３１８７（Ａ２３１８７）诱导的小鼠腹腔巨噬细胞白细胞介素６（ｉｎｔｅｒｌｅｕｋｉｎ６，ＩＬ６）ｍＲＮＡ表达水平的影响。方法：用半定量ｍＲＮＡ的ＲＴＰＣＲ。结果：异丹叶大黄素和白藜芦醇在２×１０－６和２×１０－５ｍｏｌ·Ｌ－１浓度下，均能抑制ｆＭＬＰ和Ａ２３１８７诱导的小鼠腹腔巨噬细胞ＩＬ６ｍＲＮＡ的表达。结论：该２个化合物抑制ＩＬ６活性的作用机制之一是抑制ＩＬ６ｍＲＮＡ表达。AIM: Effects of isorhapotigenin and resveratrol on interleukin 6 mRNA expression from mouse peritoneal macrophages stimulated with fMLP and A23187 in succession were explored. METHODS: Interleukin 6 mRNA was semiquantified by RTPCR. RESULTS: Isorhapotigenin and resveratrol, at the concentrations of 210-6 and 210-5 molL-1, were shown to inhibit interleukin 6 mRNA expression from mouse peritoneal macrophages stimulated with fMLP or A23187 in succession. CONCLUSION: Inhibition of interleukin 6 mRNA expression may be the mechanism of the interleukin 6 biosynthetic inhibition of isorhapotigenin and resveratrol.

关 键 词：白细胞介素6 MRNA 白藜芦醇 异丹叶大黄素 

分 类 号：R962[医药卫生—药理学] R931.71[医药卫生—药学]