血吸虫多抗原肽疫苗的合成及生物活性  

SYNTHESES AND BIOACTIVITIES OF MULTIPLE ANTIGENIC PEPTIDE VACCINES AGAINST SCHISTOSOMIASIS

作　　者：曹胜利[1,2] 秦致辉[1,2] 蔡孟深[1,2] 石佑恩[1,2] 

机构地区：[1]北京医科大学药学院生物有机化学系 [2]同济医科大学基础医学院

出　　处：《药学学报》1999年第5期368-371,共4页Acta Pharmaceutica Sinica

基　　金：卫生部科学研究基金

摘　　要：目的：合成由寡聚赖氨酸和曼氏血吸虫２８ＫｕＧＳＴ抗原肽段２６４３（Ｐ２６）、１４１１５３（Ｐ１４１），日本血吸虫２６ＫｕＧＳＴ抗原肽段１８７２０２（Ｊ１８７）组成的血吸虫多抗原肽疫苗，通过活性试验考察其抗原性及对实验动物的保护性免疫效果。方法：用固相逐步接肽法合成多抗原肽疫苗，产物经斑点酶联免疫吸附试验测定抗原性，并在无免疫佐剂存在下接种小鼠，以日本血吸虫尾蚴攻击感染，６周后剖杀小鼠进行体内成虫和肝内虫卵计数。结果：合成多抗原肽均能与感染日本血吸虫的病人或病兔血清结合，接种（Ｐ１４１）４ＭＡＰ、（Ｊ１８７）４ＭＡＰ的昆明小鼠和接种（Ｐ２６）８ＭＡＰ的ＢＡＬＢ／ｃ小鼠，与对照组相比成虫检获数分别减少６４６％，４１３％和２８１％；每克肝脏虫卵数分别减少５４９％，４５６％和４０６％。结论：合成血吸虫多抗原肽疫苗能诱导小鼠产生抵抗日本血吸虫感染的保护性免疫力。AIM: To synthesize the multiple antigenic peptides (MAPs) consisting of the oligomeric lysine core and the antigenic peptides 2643 (P26) and 141153 (P141) derived from 28 Ku glutathione Stransferase of Schitosoma mansoni (Sm28GST) and 187202 (J187) from 26 Ku glutathione Stransferse of Schistoma japonicum (Sj26GST), and to examine their antigenicities and protective effects on experimental animals. METHODS: The MAPs have been synthesized with stepwise solidphase peptide synthesis and their antigenicities have been tested with dotELISA. Having been vaccinated with the synthetic MAPs, the mice were infected with Schistosoma japonicum cercariae and were killed 6 weeks later to recover the adult worms and the eggs in liver. RESULTS: All the synthetic MAPs could be recognized and bound by both patient and infectedrabbit sera. Furthermore, immunization with (P141)4MAP or (J187)4MAP in Kunming mice and (P26)8MAP in BALB/c mice, respectively, reduced the worm burden by 644%, 413% and 281%, and reduced eggs by 549%, 456% and 406%. CONCLUSION: The synthetic MAPs can induce mice to produce protective immunity against the challenge infection with Schistosoma japonicum cercariae.

关 键 词：血吸虫 抗原肽疫苗 合成 多肽疫苗 多抗原肽 

分 类 号：R383.24[医药卫生—医学寄生虫学] R532.210.1[医药卫生—基础医学]