海水浸泡肢体火器伤时骨骼肌组织脂质过氧化反应  

Lipid Peroxidation in RabbitsSkeletal Muscle With Firearm Wounds and Seawater Immersion

作　　者：赖西南 王丽丽[1] 陈蕾[1] 陈志强[1] 张良潮[1] 于新 黄宏[1] 李兵仓[1] 

机构地区：[1]第三军医大学野战外科研究所

出　　处：《中华骨科杂志》1999年第5期307-309,共3页Chinese Journal of Orthopaedics

摘　　要：目的观察兔肢体火器伤合并海水浸泡时，伤肢骨骼肌组织过氧化脂质与抗氧化酶活力变化的特点，为早期救治提供依据。方法用滑膛枪发射钢珠致伤兔后肢，伤后将致伤兔分为两组：一组为海水浸泡组（ＳＩＧ），即将致伤兔浸泡于粗制海盐配制的人造海水中３０分钟；另一组为单纯致伤组（ＳＷＧ），伤后不浸泡海水。伤后３、６、１２、２４小时手术切取距伤道边缘０５、１５和２５ｃｍ处肌组织，测定丙二醛（ＭＤＡ）、ＡＴＰ含量和超氧化物歧化酶（ＳＯＤ）活力。取伤前骨骼肌组织作为对照。结果伤后ＳＩＧ兔伤肢骨骼肌ＭＤＡ含量明显较伤前升高，升高幅度与距伤腔距离呈反比，并呈伤后３、６小时升高，１２小时略有下降，２４小时再次升高的趋势。ＳＷＧ的ＭＤＡ变化与ＳＩＧ相似，但升高幅度低于ＳＩＧ，两组ＳＯＤ活力和ＡＴＰ含量的改变与ＭＤＡ变化呈相反趋势。结论肢体火器伤合并海水浸泡可加重伤道周围骨骼肌组织微循环障碍和能量代谢紊乱，导致氧自由基生成增加，过氧化脂质反应增强，继发损伤明显。Abstract Objective To study the lipid peroxidation and the activity of antioxidation enzyme in the skeletal muscle in hind legs of rabbits injured by firearm and seawater immersion in order to improve the early therapeutic effect Methods The rabbitshind legs were injured by steel ball bullets shot with smoothbore gun and assigned randomly into two groups, the seawater immersion (SIG) and simple wounded group(SWG) The rabbitsin SIG were soaked in artificial seawater for 30 minutes and the wounded hind legs in SWG were left as such The skeletal muscle samples after wounding were procured at 05 cm, 15 cm and 25 cm sites away from the wound tracks edge of both groups and malondialdehyde (MDA), which is the product of lipid peroxidation, activity of superoxide dismutase (SOD) and adenosine triphosphate (ATP) were assayed The skeletal muscle sample before wounding was taken and assayed the above parameters as control Results MDA content in skeletal muscle in SIG after wounding was significantly increased as compared with that before wounding and the range of increase was negatively proportional to the distance from the tracks edgeThe increase of MDA appeared markedly at 3 hours, slightly decreased at 12 hours and again increased at 24 hours after woundingThe changing character of MDA in SWG was similar with that in SIG, but the peak value was lower than that in SIG The changing trend of SOD activity and ATP content in both groups was negative with that of MDA Conclusion As firearm wound of the extremity is combined with seawater immersion, the disturbance of the energy metabolism in the skeletal muscle surrouding the wound track may be more severe and it may induce the increase of free oxygen and aggravate the lipid peroxidation resulting in an exacerbation of secondary injury Wounds,gunshotSeawate

关 键 词：战伤 烧伤 海水浸泡 过氧化脂质 SOD 

分 类 号：R826.502[医药卫生—临床医学]