嗜水气单胞菌对四环素类和氟喹诺酮类药物的耐药性研究  

In vitro study on drug-resistance characteristics of Aeromonas hydrophila to tetracyclines and fluoroquinolones

作　　者：王美珍[1] 陈昌福[1] 刘振兴[1] 谭晶晶[1] 高宇[1] 李革雷[1] 

机构地区：[1]华中农业大学水产学院,武汉430070

出　　处：《华中农业大学学报》2011年第1期89-93,共5页Journal of Huazhong Agricultural University

基　　金：农业公益性行业科研专项(200803013)

摘　　要：在离体条件下进行患病鱼体内分离的嗜水气单胞菌3个菌株对四环素类和氟喹诺酮类药物的耐药性获得、稳定性、保存条件和交叉耐药性研究。结果表明:分别在含有盐酸多西环素、盐酸四环素、诺氟沙星和左氧氟沙星的药物培养基中连续传代9次(在28℃条件下培养72 h为1代)后,四环素类对3个菌株的最小抑菌浓度上升倍数为8～32倍,氟喹诺酮类组MIC上升倍数为125～7 997倍,且耐药获得后保持稳定。同时嗜水气单胞菌对四环素类和氟喹诺酮类药物存在交叉耐药。耐药菌4℃保存10和20 d耐药性保持稳定,30 d耐药性均有不同程度的下降。The aims of this study were to investigate the development,stability,cross-resistance and preservation of Aeromonas hydrophila resistance to tetracyclines and fluoroquinolones. A.hydrophila was grown at 28 ℃ for 72 h and used it to test for the development of resistance after 9 sequential subcultures in sub-inhibitory concentrations of two tetracyclines（doxycycline and tetracycline） and two fluoroquinolones （norfloxacin and levofloxacin）.After 9 subcultures the minimal inhibitory concentrations （MIC） to tetracyclines were 8-32 times greater than the initial values,MIC values to fluoroquinolones were 125-7 997 times greater than the initial values,and drug-resistance were stable.The doxycycline-resistant isolate was resistant to tetracycline and fluoroquinolones,and the levofloxacin-resistant isolate was resistant to tetracyclines and norfloxacin.The drug-resistance stability test indicated that long-term storage of the bacteria at 4℃ could reduce their resistance to antimicrobials.

关 键 词：嗜水气单胞菌 四环素类 氟喹诺酮类 耐药性获得 耐药稳定性 交叉耐药 保存 

分 类 号：S917.1[农业科学—水产科学]