FQ-PCR检测人肠道乳酸杆菌的研究  

Study on detecting lactobacillus quantitatively by using fluorescent quantificative PCR

作　　者：冯玉奎[1] 郑长青 孙英姿[1] 高波[1] 

机构地区：[1]济南军区青岛第二疗养院检验科,山东青岛266071 [2]401医院输血科

出　　处：《实用医药杂志》2011年第1期64-66,共3页Practical Journal of Medicine & Pharmacy

基　　金：青岛市公共领域科技支撑计划项目(09-1-1-28-nsh)

摘　　要：目的应用荧光定量PCR技术对健康人粪便内乳酸杆菌进行定量分析,建立乳酸杆菌的荧光定量PCR检测方法。方法依据乳酸杆菌16S rDNA序列设计属特异性引物,应用荧光定量PCR技术检测乳酸杆菌的16SrDNA,对粪便中的乳酸杆菌进行定量检测和分析,并和用传统方法所获得的结果进行比较。结果结果显示荧光定量PCR检测乳酸杆菌和传统方法检测乳酸杆菌之间无显著性差异(9.3±1.5 vs 8.9±1.3 copy.,P>0.05)。结论荧光定量PCR技术比传统方法特异性高、敏感性强,更省时和省力。Objective To quantitatively detect Lactobacillus in fecal samples from healthy population by using fluorescent quantificative PCR（FQ-PCR）.Methods Genus-specific primer of lactobacillus was designed for 16S rDNA-targeted PCR.Bacterial genome DNA in fecal samples was extracted from the heathy population and detected by using FQ-PCR,respectively.And obtained data were compared with that of conventional method.Results The range of bacteria by using FQ-PCR was nearly equal to that of cultivation techniques.Conclusion FQ-PCR is a method with high specificity and sensitivity,and it can be applied to the quantative detection of bacteria in the intestine tract but saving more time and labor.

关 键 词：实时荧光定量PCR 乳酸杆菌 定量检测 

分 类 号：R446.5[医药卫生—诊断学]