外周formalin刺激时大鼠脊髓灰质神经元c-Fos蛋白和NMDA受体的双标记免疫细胞化学研究(英文)  

C Fos AND NMDA RECEPTOR LIKE IMMUNOREACTIVE NEURONS IN SPINAL CORD FOLLOWING PERIPHERAL FORMALIN INJECTION:A DOUBLE IMMUNOCYTOCHEMICAL STUDY IN RATS

作　　者：王航[1] 安小敏 张瑞新 乔健天[1] 

机构地区：[1]山西医科大学神经生物学研究室

出　　处：《中国神经科学杂志》1999年第2期96-102,共7页

摘　　要：利用免疫细胞化学双标技术，研究了大鼠后脚掌注入ｆｏｒｍａｌｉｎ时脊髓ｃ－Ｆｏｓ蛋白和ＮＭＤＡ受体在脊髓灰质不同层次神经元的分布。结果表明，脊髓背角Ⅰ－Ⅱ层、Ⅴ－Ⅵ层、和Ｘ层中的Ｆｏｓ样免疫反应阳性神经元中分别有２８．６％，５８．１％和６４．８％呈现ＮＭＤＡ受体样免疫反应阳性。此结果提示长时间的外周伤害性刺激作用时，脊髓不同层次的伤害性感受神经元可能由于ＮＭＤＡ受体分布的不同而在持续痛刺激过程中起着不同的作用。The coexistence of c Fos protein and NMDA receptors in the spinal cord following peripheral formalin injection into rat hindpaw was examined by using double immunocytochemical techniques.The results showed that about 26.8%,50.1%,and 64.8% of c Fos like immunoreactive (LI) neurons in laminae Ⅰ Ⅱ,laminae Ⅴ Ⅵ,and lamina Ⅹ of the dorsal horn were also NMDA like immunoreactive neurons,suggesting that the nociceptive neurons located in different spinal laminae might be functioning differentially during long lasting peripheral noxious stimulation,owing to the differential distribution of NMDA receptors on these neurons.

关 键 词：NMDA受体 C-FOS 免疫细胞化学 FORMALIN 脊髓 

分 类 号：R338.3[医药卫生—人体生理学]