体检人员乙肝病毒标志物的定性定量对比分析  

A comparative analysis on qualitative and quantitative detection of hepatitis B markers in health examinees

作　　者：张素华[1] 解松刚[1] 

机构地区：[1]苏北人民医院临床检测中心,江苏扬州225001

出　　处：《现代医药卫生》2011年第1期12-13,共2页Journal of Modern Medicine & Health

摘　　要：目的:研究时间分辨荧光免疫分析技术(TRFIA)在体检人员乙型肝炎病毒(HBV)血清免疫学表面标志物(HbsAg、HB-sAb)检测中的应用及其与ELISA法的比较。方法:用TRFIA和ELISA法对168例体检人员血清学标本进行检测,并对结果进行分析。结果:TRFIA和ELISA法比较,HBsAg结果符合率为92.9%,经配对χ2检验,P<0.05,差异有统计学意义。HBsAb结果符合率为82.7%,P<0.05,差异有统计学意义。结论:TRFIA与ELISA法检测乙肝病毒标志物相比,其特异性和敏感性高。TRFIA法作为一种高灵敏度的方法,其定量检测结果对体检人员HBV感染的诊断、治疗,特别是疫苗接种方案的提示都将起着十分积极的作用及更可靠的依据。Objective：To explore the clinical application of time-resolved fluoroimmunoassay（TRFIA） in detecting serum HBV markers（HbsAg,HBsAb） and to compare it with enzyme linked immunosorbent assay（ELISA）.Methods：Serum samples from 168 health examinees were collected and tested for levels of serum hepatitis B virus antigen and antibody by TRFIA and ELISA respectively.Results：By comparison of two methods,the coincidence rate of HBsAg and HBsAb was 92.9% and 82.7% respectively with statistical difference（P0.05）.Conclusion：TRFIA is superior to ELISA in the specificity and sensitivity of detection of hepatitis B virus antigen and antibody.As a highly sensitive assay,the quantitative detecting results of TRFIA will play more active role in the diagnosis of HBV infection and its treatment,especially in prompting the vaccination program of the health examinees,which also provides more reliable evidence.

关 键 词：乙型肝炎病毒血清学标志物 时间分辨荧光免疫分析技术 酶联免疫吸附试验 

分 类 号：R446[医药卫生—诊断学]