中华骨髓库湖北分库志愿者HLA-A*02亚型基因多态性分析  

Analysis of HLA-A*02 gene subtype polymorphisms in Hubei branch of Chinese Bone Marrow Bank

作　　者：李王霞[1] 宁勇[1] 沈钢[2] 吴剑媚[2] 朱远雁[2] 刘光箭[2] 邹洁[2] 强文[2] 

机构地区：[1]湖北中医药大学,武汉430065 [2]武汉血液中心,武汉430030

出　　处：《中国免疫学杂志》2011年第1期41-43,49,共4页Chinese Journal of Immunology

摘　　要：目的:检测中华骨髓库湖北分库志愿者HLA-A*02基因亚型,了解其亚型基因多态性分布特点。方法:采用序列特异性寡核苷酸探针聚合酶链反应技术(PCR-SSOP)及聚合酶链反应-直接测序技术(PCR-SBT)对分库1 364名志愿者进行HLA-A高分辨率分型。结果:1 364例HLA-A基因分型结果中,共691例A*02阳性,A*02低分辨基因型频率为0.301,检出A*0201、A*0203、A*0205、A*0206、A*0207、A*0210、A*0211、A*0290,8个等位基因。其中A*0201是优势基因,A*0207、A*0206、A*0203为常见型基因,本研究人群中还检测出了东亚人群罕见型基因:A*0205、A*0211、A*0290。其基因频率分布与台湾慈济脐血库、日本、北爱尔兰、亚裔美国人比较,存在统计学差异。此人群A*02低、高分辨杂合度分别为83.4%、96.7%,其高分辨率纯合子呈现规律性的分布。结论:中华骨髓库湖北分库志愿者HLA-A*02亚型基因频率呈高度多态性,其基因多态性与台湾慈济脐血库、日本、北爱尔兰、亚裔美国人存在显著性差异。Objective：To study the characteristics of HLA-A＊02 gene subtype polymorphisms by detecting the high resolution typing HLA-A gene of populations in Chinese Bone Marrow Bank（Hubei province）.Methods：PCR-SSOP and PCR-SBT were used to analyze the high resolution typing HLA-A gene of 1 364 volunteers in Hubei province.Results：Among the tested HLA-A gene of 1 364 volunteers,A＊02 was positive in 691 individuals with 0.301 of low-resolution frequency.Eight different HLA-A＊02 alleles,A＊0201,A＊0203,A＊0205,A＊0206,A＊0207,A＊0210,A＊0211 and A＊0290,were identified in Chinese Bone Marrow Bank,of which A＊0201 was predominant allele while A＊0207,A＊0206 and A＊0203 were common alleles.Meanwhile,several rare alleles in East Asia population including A＊0205,A＊0211 and A＊0290 were also identified.There was a significant difference in the frequency distribution of HLA-A＊02 gene when compared with Taiwan Tzu Chi Cord Blood Bank,Japanese,Ireland Northern and USA Asian.The heterozygosity of A＊02 of populations in Chinese Bone Marrow Bank（Hubei province）was 83.4% at highresolution level,96.7% at low-resolution levels,respectively,and the distribution of A＊02 homozygote at high-resolution level was regular.Conclusion：A high polymorphism of HLA-A＊02 gene frequency is present in Chinese Bone Marrow Bank（Hubei province）,with significant difference from those in Taiwan Tzu Chi Cord Blood Bank,Japanese,Ireland Northern and USA Asians.

关 键 词：骨髓 HLA-A＊02 基因多态性 基因频率 

分 类 号：R457.11[医药卫生—治疗学]