中国HIV感染长期不进展者T细胞表面IL-7受体表达研究  

Study on the expression of the IL-7 receptor on T lymphocytes in HIV infected Long-Term Non-Progressors

作　　者：李革飞[1] 张子宁[1] 张旻[1] 刘静[1] 施万英[1] 金鑫[1] 姜拥军[1] 王亚男[1] 尚红[1] 

机构地区：[1]中国医科大学附属第一医院卫生部艾滋病免疫学重点实验室,沈阳110001

出　　处：《中国免疫学杂志》2011年第1期60-63,69,共5页Chinese Journal of Immunology

基　　金：国家科技重大专项课题资助项目(2008ZX10001-001);国家重点基础研究发展计划(973计划)(2006CB504206);辽宁省高等学校攀登学者(辽教发[2008]90号);辽宁省重点实验室项目(2008S242)资助

摘　　要：目的:了解中国HIV感染长期不进展者(Long-Term Non-Progressor,LTNP)T细胞表面IL-7(Interleukin-7,IL-7)受体的表达,分析其与疾病进展的关系。方法:收集LTNP组、HIV组、AIDS组及健康对照组(每组各20例)的抗凝全血,用流式细胞仪检测IL-7受体的表达,并分析IL-7受体表达与血浆中IL-7水平的相关性。结果:LTNP组CD4+T、CD8+T、中枢记忆T细胞(Central memory T cells,Tcm)、效应记忆性T细胞(Effector memory T cells,Tem)表面IL-7受体表达水平明显高于无症状HIV感染组(HIV组)、AIDS病人组(AIDS组)(P<0.05),LTNP组初始T细胞(Na ve T cells,Na ve)、终末分化效应记忆型T细胞(Terminallydifferentiated effector memory T cells,Tem/td)表面IL-7受体表达水平与HIV组、AIDS组间差异无统计学意义。HIV/AIDS患者IL-7受体在CD4+T和CD8+T细胞上表达的百分率与血浆IL-7水平呈明显负相关(P<0.05),与CD4+T细胞数量呈明显的正相关(P<0.05),与病毒载量呈明显负相关(P<0.05)。结论:外周血T细胞表面IL-7受体表达与HIV疾病进展密切相关,LTNP组T细胞保持较高水平的IL-7受体表达,可能是保护因素之一。Objective：To investigate the expression of Interleukin-7（IL-7） receptor on T lymphocytes of Long-Term Non-Progressors（LTNP）and its association with disease progression.Methods：LTNP,HIV,AIDS patients and healthy controls（20 patients for each group） were enrolled and flow cytometry was used to detect the IL-7 receptor expression on T lymphocytes of whole blood samples taken from the patients and the controls.The association between IL-7 receptor expression on T lymphocytes and IL-7 level in plasma was analyzed.Results：The expression of IL-7 receptor on CD4 and CD8＋T lymphocytes and central memory T cells（Tcm）,effector memory T cells（Tem） of LTNP group was significantly higher than that of AIDS and HIV groups（P〈0.05）;The expression of IL-7 receptor on Nave and Terminally differentiated effector memory T cells（Tem/td） of LTNP group was not statistically different from that of AIDS,HIV groups and healthy controls.The percentage of IL-7 receptor expression on CD4,CD8＋T cells of HIV/AIDS patients negatively correlated with IL-7 level in plasma and HIV virus load and positively correlated with CD4 absolute value（P〈0.05）.Conclusion：The expression of the IL-7 receptor on T lymphocytes from peripheral blood is significantly correlated with HIV disease progression and higher level of IL-7 receptor expression on T cells of LTNPs may play a role in slowing down the disease progression.

关 键 词：人类免疫缺陷病毒 IL-7受体 长期不进展 

分 类 号：R512.91[医药卫生—内科学]