紫叶稠李叶片离体培养再生植株  

Study on Regeneration Plant of Prunus Wilsonil Leaves in vitro

作　　者：邢国杰[1] 高明[1] 谭化[1] 李俊波[1] 崔云志[1] 王中伟[1] 

机构地区：[1]吉林省农业科学院生物技术中心,吉林长春130124

出　　处：《北方园艺》2011年第3期166-168,共3页Northern Horticulture

摘　　要：以紫叶稠李为试材,采用组织培养方法,研究不同激素对其试管苗不定芽诱导、不定芽生根及成苗影响。结果表明:叶片切块可诱导产生大量的不定芽,MS附加0.5mg/L BA和0.01mg/L NAA的培养基不定芽诱导率为50.1%;利用植物激素0.5mg/L BA,0.5mg/LNAA,0.5mg/L KT和0.2mg/L IBA的组合,不定芽可多次继代培养增殖;不定芽在附加0.4mg/L IBA的MS培养基上生根,生根率为67%。该试验结果说明,紫叶稠李试管苗叶片可作为外植体离体培养获得再生植株,BA是主要的调控植物激素,IBA对生根有一定促进作用。Rapid propagation of Prunus Wilsonil was preliminary stuidied using leaves of testtube seedling for in vitro culture.The results showed that the a chip of leaf from test-tube plantlet were cultured,a mass of adventitious shoots were induced with MS medium containing BA 0.5 mg/L ＋ NAA 0.01 mg/L,its induction rate was 50.1%;and combination with BA 0.5 mg/L ＋ NAA 0.5 mg/L ＋ KT 0.5 mg/L ＋ IBA 0.2 mg/L,adventitious shoots could be used repeatedly to subculture proliferation：rooting was induced with adventitious shoots in MS supplemented of IBA 0.4 mg/L,rooting rate was 67%.Thus the leaf of testtube seedling can be used as explant for rapid propagation of Prunus Wilsonil.

关 键 词：紫叶稠李 不定芽 离体再生 

分 类 号：S687[农业科学—观赏园艺]