葫芦素B半乳糖化肝靶向脂质体体外溶血性的研究  

Study on Hemolysis of the Cucurbitacin B Galactosylated Liver-Targeting Liposomes in Vitro

作　　者：董晓辉[1] 李乐[2] 石莉[2] 张玲[1] 卢懿[1] 邓意辉[1] 

机构地区：[1]沈阳药科大学,沈阳110016 [2]辽宁省药物研究院,沈阳110015

出　　处：《中国药学杂志》2011年第3期194-197,共4页Chinese Pharmaceutical Journal

摘　　要：目的研究不同处方的葫芦素B半乳糖化肝靶向脂质体的体外溶血性。方法通过常规体外试管法并结合显微镜下的红细胞形态学观察对3个处方的葫芦素B半乳糖化肝靶向脂质体的溶血作用进行评价,并通过筛选合适的破乳剂,应用分光光度法于575 nm下测定其溶血度。结果肉眼观察3个葫芦素B半乳糖化肝靶向脂质体处方均无明显溶血现象,以10%Triton X-100为破乳剂通过分光光度法测定各处方的溶血度均小于5%。结论3个葫芦素B半乳糖化肝靶向脂质体处方的溶血性符合注射剂要求,常规体外试管法同显微观察及分光光度法联合使用,可更准确地对制剂的溶血性进行判断,10%Triton X-100可做为脂质体溶血实验的优良破乳剂。OBJECTIVE To investigate hemolysis caused by the interaction between rabbit erythrocytes and cucurbitacin B galactosylated liver-targeting liposomes prepared with different adjuvants. METHODS The hemolytic phenomena of the three cucurbitacin B galactosylated liver-targeting liposomes formulations were observed by macroscopic observation and the optical microscope photos of the rabbit erythrocytes were taken with digital microscope. After being treated with a proper emulsion-breaker, the hemolysis percentage of rabbit erythrocytes induced by liposomes were spectrophotometrically determined at 575 nm. RESULTS The three formulations of eueurhitaein B galaetosylated liver-targeting liposomes with different adjuvants did not show any hemolytic behavior. The hemolysis percentage of the three formulations were all below 5 % by using 10% Triton X-100 as the emulsion-breaker. CONCLUSION Three eucurhitacin B galactosylated liver-targeting liposomes formulations are injectable. The union of macroscopic observation, the optical microscope observation and the spectrophotometry are reliable and feasible to evaluate the hemolytic behavior. 10% Triton X-100 can be used in the hemolysis test as a good emulsion-breaker for liposomes.

关 键 词：葫芦素B 半乳糖化肝靶向脂质体 溶血 常规体外试管法 红细胞形态学 分光光度法 

分 类 号：R944[医药卫生—药剂学]