切花菊高效不定芽再生体系的建立  

Efficient shoots regeneration from adventitious buds of cutting chrysanthemum

作　　者：刘冰[1] 杨际双[2] 肖建忠[1] 黄兆平[1] 李单[1] 

机构地区：[1]河北农业大学园林与旅游学院,河北保定071001 [2]河北农业大学园艺学院,河北保定071001

出　　处：《河北农业大学学报》2011年第1期25-29,共5页Journal of Hebei Agricultural University

基　　金：河北农业大学留学回国人员科研启动基金(2004-897)

摘　　要：以"神马"和"出水芙蓉"2个切花菊品种的叶片为外植体,来建立不定芽再生体系。结果表明:基因型和植物生长调节剂是影响叶片不定芽再生的重要因素,并且较高浓度生长调节剂会导致不定芽和再生小苗玻璃化,不利于植株再生。"神马"的最适直接诱导不定芽再生培养基为MS+NAA 1 mg/L+6-BA 1 mg/L,再生率高达95.5%;"出水芙蓉"的最适直接诱导不定芽再生培养基为MS+NAA 0.1 mg/L+6-BA 1 mg/L,但再生率仅为46.3%。植物生长调节剂影响再生植株生根数,适宜的生根培养基为1/2 MS+0.7 mg/L NAA。Using leaf disks of cutting chrysanthemum（＂Jinba＂ and ＂Chushui Furong＂）as explants,an efficient regeneration system from the adventitious buds was established.The results indicated that the genotypes and plant growth regulators greatly affected shoot regeneration from adventitious buds.High concentrations of the plant growth regulators increased the vitrification of adventitious buds and shoots from leaves,and then,decreased the shoot regeneration.The optimum medium which could directly induce shoot regeneration of ＂Jinba＂ was MS medium supplemented with 1.0 mg/L naphthalene-acetic acid（NAA） and 1.0 mg/L 6-benzylamino purine（6-BA）,on which the regeneration rate was 95.5%.In ＂Chushui Furong＂,that was MS medium with 0.1 mg/L NAA and 1.0 mg/L 6-BA,and the regeneration rate was 46.3%.The plant growth regulators affected the No.of roots induction from shoots obviously,and the optimum rooting medium was 1/2 MS medium with 0.7 mg/L NAA.

关 键 词：切花菊 不定芽 再生 叶盘 

分 类 号：S682.11[农业科学—观赏园艺]