5-HT及5-HT_(1A)受体在不同妊娠阶段大鼠子宫中的分布  

Distribution of 5-HT and 5-HT_(1A) Receptors in Uterus of Rats During Pregnancy

作　　者：郝建军[1] 徐永平[1] 王红梅[1] 胡小海[1] 多吉拉姆[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《中国农业科学》2011年第3期604-612,共9页Scientia Agricultura Sinica

基　　金：陕西省"奶牛乳房炎技术"支撑与示范项目(14220201)

摘　　要：【目的】研究5-羟色胺(5-hydroxytryptamine,5-HT)及5-HT1A受体在SD大鼠妊娠期子宫中的分布情况和变化规律。【方法】选取均为正常妊娠的30只雌性SD大鼠分为妊娠3 d、妊娠5 d、妊娠7 d、妊娠13 d、妊娠19 d及产后第1天6组,处死各组大鼠,取子宫制作石蜡切片,经免疫组织化学SP法染色后,进行观察分析。【结果】5-HT及5-HT1A受体免疫阳性产物广泛存在于子宫内膜上皮细胞、子宫腺上皮细胞、血管内皮细胞、基质细胞、蜕膜细胞、免疫细胞及肌纤维中;5-HT在妊娠7 d和妊娠13 d着色最深,5-HT1A受体在妊娠13 d着色最深。5-HT及5-HT1A受体免疫阳性产物相对表达量的T检验表明,子宫内膜5-HT在妊娠7 d和妊娠13 d的表达较妊娠3 d、妊娠5 d和产后极显著的增加(P<0.01),较妊娠19 d显著增加(P<0.05);子宫肌层5-HT在妊娠19 d的表达较妊娠7 d显著减少(P<0.05),较妊娠13 d极显著的减少(P<0.01)。子宫内膜5-HT1A受体在妊娠13 d较妊娠3 d、妊娠5 d、妊娠19 d及产后极显著的增加(P<0.01),较妊娠7 d显著增加(P<0.05);子宫肌层5-HT1A受体在妊娠13 d较妊娠各个时期极显著增加(P<0.01)。5-HT和5-HT1A受体在妊娠期子宫中的表达,具有极显著的正相关性。【结论】5-HT和5-HT1A受体在妊娠期子宫中的表达具有一定规律,5-HT通过5-HT1A受体参与妊娠的调节。【Objective】The distribution and expression variation of 5-HT and 5-HT1A receptors in the uterus of the Sprague Dawley during pregnancy were studied.【Method】Thirty mature female SD rats with normal pregnancy were randomly divided into 6 groups： pregnancy 3 days group（3D）,pregnancy 5 days group（5D）,pregnancy 7 days group（7D）,pregnancy13 days group（13D）,pregnancy 19 days group（19D） and the first day of postpartum group（P1）.The expression of 5-HT and 5-HT1A R in uterus of the rats during pregnancy cycle were studied by immunohistochemical SP method.【Result】The 5-HT and 5-HT1A R immunoreactive products mainly located in endometrial epithelial cells,the glandular epithelium,vascular endothelial cells,stromal cells,decidual cells,immune cells and muscle fiber.The strongest coloration of 5-HT was in 7D and 13D.The strongest coloration of 5-HT1A R was in 13D.The relative expression of immunoreactive product 5-HT and 5-HT1A R of T-test showed that,compared with the 3D,5D and P1,the expression of 5-HT in 7D and 13D of endometrium was extremely significantly increased（P0.01）,compared with the 19D,the expression of 5-HT in 7D and 13D were significantly increased（P0.05）.Compared with the 7D,the expression of 5-HT in 19D of myometrium was significantly decreased（P0.05）,compared with the 13D,the expression of 5-HT in 19D of myometrium was extremely significantly decreased（P0.01）.Compared with the 3D,5D,7D and P0,the expression of 5-HT1A R in 13D of endometrium was extremely significantly increased（P0.01）,Compared with the 7D,expression of 5-HT1A R in 13D of endometrium was significantly increased（P0.05）.Compared with the 3D,5D,7D,19D and P1,the expression of 5-HT1A R in 13D of myometrium was extremely significantly increased（P0.01）.Expression of 5-HT and 5-HT1AR in pregnancy had highly significant positive correlation.【Conclusion】The expression of 5-HT and 5-HT1A R in the uterus in different pregnancy periods was found to adapt to some certain rules,5-HT throu

关 键 词：5-羟色胺 5-HT1A受体 免疫组织化学SP法 妊娠期 子宫 SD大鼠 

分 类 号：S865.12[农业科学—野生动物驯养]