葛根种质资源的分子地理标识研究  

Classification of Pueraria lobata in different geographical regions

作　　者：刘东吉[1] 余智奎[1] 刘春生[1] 王学勇[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《中国中药杂志》2011年第3期299-301,共3页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30672615)

摘　　要：目的:利用葛根的ITS,psbK-psbI,trnH-psbA序列信息对不同产地葛根进行分类,为分析不同产地的葛根药材提供依据。方法:采集东北、华北、华中和西北共8个居群24份葛根样品,提取DNA,扩增ITS序列、psbK-psbI及trnH-psbA序列、测序,通过序列比对,根据差异划分基因型或单倍型。结果:ITS1,ITS2序列各有1个单碱基突变,5.8S序列有1个单碱基突变;psbK-psbI序列有2个单碱基突变;trnH-psbA序列有1个单碱基突变,还有1段10个碱基的缺失突变。结论:利用ITS序列将葛根划分成4个基因型,根据psbK-psbI和trnH-psbA序列均将葛根划分成2个单倍型,为进一步评价提供依据。Objective： To classify Pueraria lobata originated from different geographical regions based on ITS,psbK-psbI and trnH-psbA information. Method： Twenty-four samples of P. lobata were collected from northeast China, north China, central China and northwest China. DNA extraction, PCR,sequence and genotypes/haplotypes analysis were performed . Result： ITS1, 5.8S and ITS2 varied only 1 bp respectively, psbK-psbI 2 bps; trnH-psbA varied 1 bp and 10 bp deletion. Conclusion： Based on the variation of ITS,psbK-psbI and trnH-psbA, 4 genotypes and 2 haplotypes were identified, respectively.

关 键 词：葛根 不同产地 分类 

分 类 号：S567.239[农业科学—中草药栽培]